Literature DB >> 2113898

Purification and properties of a thermophilic and thermostable DNA polymerase from the archaebacterium Sulfolobus solfataricus.

R Rella1, C A Raia, F M Pisani, S D'Auria, R Nucci, A Gambacorta, M De Rosa, M Rossi.   

Abstract

A DNA-dependent DNA polymerase was obtained in homogenous form from the thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme, purified 706-fold, has a molecular mass of about 110000 daltons as determined by gel filtration and by glycerol gradient centrifugation. It requires Mg++ for its activity and has a pH optimum of 7.7. The activity is sharply dependent on the ionic strength. The enzyme is thermostable; its properties and activity requirements were characterized. The features of this enzyme are compared to those of other DNA polymerases isolated either from prokaryotes or eukaryotes.

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Year:  1990        PMID： 2113898

Source DB:  PubMed          Journal:  Ital J Biochem        ISSN： 0021-2938


  6 in total

1.  A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases.

Authors:  F M Pisani; C De Martino; M Rossi
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

2.  Heat-stable enzymes from extremely thermophilic and hyperthermophilic microorganisms.

Authors:  C Leuschner; G Antranikian
Journal:  World J Microbiol Biotechnol       Date:  1995-01       Impact factor: 3.312

3.  Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus.

Authors:  T Uemori; Y Ishino; H Toh; K Asada; I Kato
Journal:  Nucleic Acids Res       Date:  1993-01-25       Impact factor: 16.971

4.  Expression of the thermostable beta-galactosidase gene from the archaebacterium Sulfolobus solfataricus in Saccharomyces cerevisiae and characterization of a new inducible promoter for heterologous expression.

Authors:  M Moracci; A La Volpe; J F Pulitzer; M Rossi; M Ciaramella
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490

5.  Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme.

Authors:  Jiangyu Yan; Thomas R Beattie; Adriana L Rojas; Kelly Schermerhorn; Tamzin Gristwood; Jonathan C Trinidad; Sonja V Albers; Pietro Roversi; Andrew F Gardner; Nicola G A Abrescia; Stephen D Bell
Journal:  Nat Commun       Date:  2017-05-02       Impact factor: 14.919

6.  A trimeric DNA polymerase complex increases the native replication processivity.

Authors:  Andrey L Mikheikin; Hsiang-Kai Lin; Preeti Mehta; Linda Jen-Jacobson; Michael A Trakselis
Journal:  Nucleic Acids Res       Date:  2009-11       Impact factor: 16.971
  6 in total

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