Literature DB >> 2113736

Three-dimensional identification of actin filaments in phalloidin-treated rat livers by quick-freezing and deep-etching method.

A Naramoto1, S Ohno, N Itoh, H Takami, K Nakazawa, H Shigematsu.   

Abstract

An increase in microfilaments in phalloidin-treated hepatocytes of Wistar rats was identified three-dimensionally with myosin subfragment 1 (S1) on replica membranes, using the quick-freezing and deep-etching method. Almost all of the reticular microfilaments around the bile canaliculi and beneath the lateral cell membranes were decorated on their surfaces by S1 attachment. Some showed periodic structures. However, thinner filaments with diameters of 4-7 nm were not decorated by S1. Bundled intermediate filaments around the bile canalicular microfilaments and intermediate filaments localized among cell organelles had smooth surfaces without S1 decoration. The microfilaments decorated by S1 were attached directly to bundled intermediate filaments. The quick-freezing and deep-etching method is useful in analysing cytoskeletal pathology and can be applied to histochemical fields.

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Year:  1990        PMID： 2113736     DOI： 10.1007/bf01600104

Source DB:  PubMed          Journal:  Virchows Arch A Pathol Anat Histopathol        ISSN： 0174-7398


  13 in total

1.  Cytokeratin intermediate filaments of rat hepatocytes: different cytoskeletal domains and their three-dimensional structure.

Authors:  Y Katsuma; N Marceau; M Ohta; S W French
Journal:  Hepatology       Date:  1988 May-Jun       Impact factor: 17.425

2.  Reaction of human smooth muscle antibody with liver cells.

Authors:  L J Farrow; E J Holborow; W D Brighton
Journal:  Nat New Biol       Date:  1971-08-11

3.  Pathology of cytoskeleton of liver cells: demonstration of mallory bodies (alcoholic hyalin) in murine and human hepatocytes by immunofluorescence microscopy using antibodies to cytokeratin polypeptides from hepatocytes.

Authors:  H Denk; W W Franke; B Dragosics; I Zeiler
Journal:  Hepatology       Date:  1981 Jan-Feb       Impact factor: 17.425

Review 4.  Fine (2-5-nm) filaments: new types of cytoskeletal structures.

Authors:  T M Roberts
Journal:  Cell Motil Cytoskeleton       Date:  1987

5.  Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures.

Authors:  M Osborn; K Weber
Journal:  Methods Cell Biol       Date:  1982       Impact factor: 1.441

6.  Influence of colchicine and phalloidin on bile secretion and hepatic ultrastructure in the rat. Possible interaction between microtubules and microfilaments.

Authors:  M Dubin; M Maurice; G Feldmann; S Erlinger
Journal:  Gastroenterology       Date:  1980-10       Impact factor: 22.682

7.  Fluorescent staining of microfilaments with heavy meromyosin labeled with N-(7-dimethylamino-4-methylcoumarinyl) maleimide.

Authors:  T Namihisa; K Tamura; K Saifuku; H Imanari; H Kuroda; Y Kanaoka; Y Okamoto; T Sekine
Journal:  J Histochem Cytochem       Date:  1980-04       Impact factor: 2.479

8.  Pathologic changes in the cytokeratin pericanalicular sheath in experimental cholestasis and alcoholic fatty liver.

Authors:  M Ohta; N Marceau; S W French
Journal:  Lab Invest       Date:  1988-07       Impact factor: 5.662

9.  Formation of arrowhead complexes with heavy meromyosin in a variety of cell types.

Authors:  H Ishikawa; R Bischoff; H Holtzer
Journal:  J Cell Biol       Date:  1969-11       Impact factor: 10.539

10.  Filament organization revealed in platinum replicas of freeze-dried cytoskeletons.

Authors:  J E Heuser; M W Kirschner
Journal:  J Cell Biol       Date:  1980-07       Impact factor: 10.539
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  7 in total

1.  Three-dimensional studies of the cytoskeleton of cultured hepatocytes: a quick-freezing and deep-etching study.

Authors:  S Ohno; Y Fujii
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1991

2.  Three-dimensional ultrastructure of glomerular injury in serum sickness nephritis using the quick-freezing and deep-etching method.

Authors:  A Naramoto; S Ohno; K Nakazawa; H Takami; N Itoh; H Shigematsu
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1991

3.  Immunocytochemical study of dystrophin in cultured mouse muscle cells by the quick-freezing and deep-etching method.

Authors:  Y C Park-Matsumoto; S Ohno; T Baba; T Kobayashi; H Tsukagoshi
Journal:  Histochem J       Date:  1992-06

4.  Ultrastructural study of Cryptococcus neoformans by quick-freezing and deep-etching method.

Authors:  N Sakaguchi; T Baba; M Fukuzawa; S Ohno
Journal:  Mycopathologia       Date:  1993-03       Impact factor: 2.574

5.  Localization of laminin in nephritic glomeruli as revealed by a quick-freezing and deep-etching method with immunohistochemistry.

Authors:  A Naramoto; S Ohno; N Itoh; N Shibata; H Shigematsu
Journal:  Histochem J       Date:  1992-10

6.  Ultrastructure of matriceal changes in chronic phase of Masugi nephritis by quick-freezing and deep-etching method.

Authors:  A Naramoto; S Ohno; N Itoh; N Shibata; K Nakazawa; H Takami; H J Duan; H Kasahara; H Shigematsu
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1991

7.  Three-dimensional ultrastructure of anionic sites of the glomerular basement membrane by a quick-freezing and deep-etching method using a cationic tracer.

Authors:  A Yoshimura; S Ohno; K Nakano; H Oniki; K Inui; T Ideura; S Koshikawa
Journal:  Histochemistry       Date:  1991
  7 in total

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