OBJECTIVE: To evaluate the recombinant membranous protein 29000 (rSj29) of Schistosoma japonicum in the immunodiagnosis of schistosomiasis by indirect ELISA. METHODS: 394 serum samples collected from two low-endemic villages in Anhui Province were tested by rSj29-ELISA and AWA-ELISA using adult worm antigen (AWA) of S. japonicum. Meanwhile all subjects were each tested by 9 examinations with 3 fecal samples by modified Kato-Katz method, and by indirect hemagglutination assay (IHA). RESULTS: It was found that the positive detection rates of stool examination, IHA, rSj29-ELISA and AWA-ELISA were 4.8% (19/394), 62.2% (245/394), 68.3% (269/394), and 89.9% (354/394), respectively. There was no statistical difference between rSj29-ELISA and IHA (chi2 = 3.2, P > 0.05) with the coincidence of 80.7% (318/394). The coincidence of IHA, rSj29-ELISA and AWA-ELISA with stool examination was 42.6% (168/394), 36.0% (142/394), and 15.0% (59/394), respectively. CONCLUSION: The indirect ELISA with rSj29 antigen shows similar effect as IHA for the diagnosis of schistosomiasis.
OBJECTIVE: To evaluate the recombinant membranous protein 29000 (rSj29) of Schistosoma japonicum in the immunodiagnosis of schistosomiasis by indirect ELISA. METHODS: 394 serum samples collected from two low-endemic villages in Anhui Province were tested by rSj29-ELISA and AWA-ELISA using adult worm antigen (AWA) of S. japonicum. Meanwhile all subjects were each tested by 9 examinations with 3 fecal samples by modified Kato-Katz method, and by indirect hemagglutination assay (IHA). RESULTS: It was found that the positive detection rates of stool examination, IHA, rSj29-ELISA and AWA-ELISA were 4.8% (19/394), 62.2% (245/394), 68.3% (269/394), and 89.9% (354/394), respectively. There was no statistical difference between rSj29-ELISA and IHA (chi2 = 3.2, P > 0.05) with the coincidence of 80.7% (318/394). The coincidence of IHA, rSj29-ELISA and AWA-ELISA with stool examination was 42.6% (168/394), 36.0% (142/394), and 15.0% (59/394), respectively. CONCLUSION: The indirect ELISA with rSj29 antigen shows similar effect as IHA for the diagnosis of schistosomiasis.