PURPOSE: We engineered a flexible fiber-optic microendoscope for longitudinal optical imaging studies in a mouse model of disseminated ovarian cancer. PROCEDURES: The microendoscope delivers 470 nm excitation light from a light-emitting diode through a fiber-optic bundle with outer diameter of 680 μm. Optics were optimized to maximize power and lateral resolution. We used this instrument to repetitively monitor intraperitoneal growth of HeyA8 ovarian cancer cells stably transduced with green fluorescent protein over 4 weeks. RESULTS: The microendoscope achieves 0.7 mW power and lateral resolution of 4 μm. Initial in vivo imaging studies visualized single cells and small clusters of malignant cells with subsequent studies showing tumor masses and vasculature. We also resolved single cells within intraperitoneal tumor masses. CONCLUSIONS: These studies establish microendoscope technology with single cell resolution for minimally-invasive, longitudinal imaging in living animals. This technology will advance future molecular imaging studies of ovarian cancer and other diseases.
PURPOSE: We engineered a flexible fiber-optic microendoscope for longitudinal optical imaging studies in a mouse model of disseminated ovarian cancer. PROCEDURES: The microendoscope delivers 470 nm excitation light from a light-emitting diode through a fiber-optic bundle with outer diameter of 680 μm. Optics were optimized to maximize power and lateral resolution. We used this instrument to repetitively monitor intraperitoneal growth of HeyA8 ovarian cancer cells stably transduced with green fluorescent protein over 4 weeks. RESULTS: The microendoscope achieves 0.7 mW power and lateral resolution of 4 μm. Initial in vivo imaging studies visualized single cells and small clusters of malignant cells with subsequent studies showing tumor masses and vasculature. We also resolved single cells within intraperitoneal tumor masses. CONCLUSIONS: These studies establish microendoscope technology with single cell resolution for minimally-invasive, longitudinal imaging in living animals. This technology will advance future molecular imaging studies of ovarian cancer and other diseases.
Authors: Tanjew Dittgen; Axel Nimmerjahn; Shoji Komai; Pawel Licznerski; Jack Waters; Troy W Margrie; Fritjof Helmchen; Winfried Denk; Michael Brecht; Pavel Osten Journal: Proc Natl Acad Sci U S A Date: 2004-12-17 Impact factor: 11.205
Authors: Pei-Lin Hsiung; Pei-Lei Hsiung; Jonathan Hardy; Shai Friedland; Roy Soetikno; Christine B Du; Amy P Wu; Peyman Sahbaie; James M Crawford; Anson W Lowe; Christopher H Contag; Thomas D Wang Journal: Nat Med Date: 2008-03-16 Impact factor: 53.440
Authors: Martin Goetz; Alex Ziebart; Sebastian Foersch; Michael Vieth; Maximilian J Waldner; Peter Delaney; Peter R Galle; Markus F Neurath; Ralf Kiesslich Journal: Gastroenterology Date: 2009-10-21 Impact factor: 22.682
Authors: Timothy J Muldoon; Mark C Pierce; Dawn L Nida; Michelle D Williams; Ann Gillenwater; Rebecca Richards-Kortum Journal: Opt Express Date: 2007-12-10 Impact factor: 3.894
Authors: Jennifer M Watson; Photini F Rice; Samuel L Marion; Molly A Brewer; John R Davis; Jeffrey J Rodriguez; Urs Utzinger; Patricia B Hoyer; Jennifer K Barton Journal: J Biomed Opt Date: 2012-07 Impact factor: 3.170