OBJECTIVE: Lectin-like oxidized LDL receptor-1 (LOX-1), the endothelial receptor for OxLDL, is believed to be responsible for a number of OxLDL-induced effects in the endothelium. METHODS AND RESULTS: In the present study we showed that LDL modified by 15-lipoxygenase (15LO-LDL), a form of minimally modified lipoprotein, beside its ability to induce pro-inflammatory responses such as oxidative stress and the expression of adhesion molecules, significantly increases LOX-1 expression in endothelial cells, both at transcriptional and at protein level. Such effect is likely to be mediated by p38 MAPK and NF-kB pathways. We then permanently overexpressed LOX-1 in an endothelial cell line and showed that 15LO-LDL were a ligand for LOX-1, and that the interaction LOX-1/15LO-LDL upregulated ICAM-1 surface expression. CONCLUSION: Altogether these results indicate minimally modified LDL as a new inducer for LOX-1 expression and as a new ligand for LOX-1.
OBJECTIVE:Lectin-like oxidized LDL receptor-1 (LOX-1), the endothelial receptor for OxLDL, is believed to be responsible for a number of OxLDL-induced effects in the endothelium. METHODS AND RESULTS: In the present study we showed that LDL modified by 15-lipoxygenase (15LO-LDL), a form of minimally modified lipoprotein, beside its ability to induce pro-inflammatory responses such as oxidative stress and the expression of adhesion molecules, significantly increases LOX-1 expression in endothelial cells, both at transcriptional and at protein level. Such effect is likely to be mediated by p38 MAPK and NF-kB pathways. We then permanently overexpressed LOX-1 in an endothelial cell line and showed that 15LO-LDL were a ligand for LOX-1, and that the interaction LOX-1/15LO-LDL upregulated ICAM-1 surface expression. CONCLUSION: Altogether these results indicate minimally modified LDL as a new inducer for LOX-1 expression and as a new ligand for LOX-1.
Authors: Iryna Voloshyna; Sony Modayil; Michael J Littlefield; Elise Belilos; Kristina Belostocki; Lois Bonetti; Gary Rosenblum; Steven E Carsons; Allison B Reiss Journal: Exp Biol Med (Maywood) Date: 2013-09-02
Authors: Iryna Voloshyna; Isaac Teboul; Michael J Littlefield; Nicolle M Siegart; George K Turi; Melissa J Fazzari; Steven E Carsons; Joshua DeLeon; Allison B Reiss Journal: Exp Biol Med (Maywood) Date: 2016-04-27
Authors: Jaume Padilla; Nathan T Jenkins; Sewon Lee; Hanrui Zhang; Jian Cui; Mozow Y Zuidema; Cuihua Zhang; Michael A Hill; James W Perfield; Jamal A Ibdah; Frank W Booth; J Wade Davis; M Harold Laughlin; R Scott Rector Journal: Physiol Genomics Date: 2013-04-16 Impact factor: 3.107