PURPOSE: The purpose of the study was to sort side population (SP) cells from the neuroblastoma SK-N-SH cell line and to systematically investigate whether this population has stem cell characteristics. METHODS: Side population and non-SP cells were separated from the SK-N-SH cell line by flow cytometry, and their morphologic characteristics were analyzed by light and electron microscopy. We also used Western blotting to analyze marker proteins, Cell Counting Kit-8 assay for proliferative ability, series differentiation studies for differentiation properties, and Matrigel invasion study and tumorigenicity assay for malignant potential. RESULTS: The SK-N-SH SP cells expressed high levels of stem cell markers, had high proliferative and malignant abilities, and had the capacity for rapid differentiation. The non-SP cells expressed differentiated cell marker proteins at high levels, had low proliferative and malignant abilities, and exhibited slow differentiation. CONCLUSIONS: The SK-N-SH SP cells have cancer stem cell-like properties.
PURPOSE: The purpose of the study was to sort side population (SP) cells from the neuroblastoma SK-N-SH cell line and to systematically investigate whether this population has stem cell characteristics. METHODS: Side population and non-SP cells were separated from the SK-N-SH cell line by flow cytometry, and their morphologic characteristics were analyzed by light and electron microscopy. We also used Western blotting to analyze marker proteins, Cell Counting Kit-8 assay for proliferative ability, series differentiation studies for differentiation properties, and Matrigel invasion study and tumorigenicity assay for malignant potential. RESULTS: The SK-N-SH SP cells expressed high levels of stem cell markers, had high proliferative and malignant abilities, and had the capacity for rapid differentiation. The non-SP cells expressed differentiated cell marker proteins at high levels, had low proliferative and malignant abilities, and exhibited slow differentiation. CONCLUSIONS: The SK-N-SH SP cells have cancer stem cell-like properties.
Authors: Sheron Campos Cogo; Thatyanne Gradowski Farias da Costa do Nascimento; Fernanda de Almeida Brehm Pinhatti; Nilton de França Junior; Bruna Santos Rodrigues; Luciane Regina Cavalli; Selene Elifio-Esposito Journal: Exp Biol Med (Maywood) Date: 2020-08-12
Authors: Jeremy T Baeten; Michael R Waarts; Margaret M Pruitt; Wen-Ching Chan; Jorge Andrade; Jill L O de Jong Journal: Haematologica Date: 2019-01-10 Impact factor: 9.941
Authors: Timofey Lebedev; Anton Buzdin; Elmira Khabusheva; Pavel Spirin; Maria Suntsova; Maxim Sorokin; Vladimir Popenko; Petr Rubtsov; Vladimir Prassolov Journal: Int J Mol Sci Date: 2022-07-13 Impact factor: 6.208