Literature DB >> 2112525

A simple, general method for detecting retroviral RNAs expressed in cells.

F Harada1, Y Hirose, M Tanaka, T Sasaki, H Kamiya, K Miura.   

Abstract

A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A+ RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3'-terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV-I and HTLV-II in virus-infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)-derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.

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Year:  1990        PMID： 2112525      PMCID： PMC5918037          DOI： 10.1111/j.1349-7006.1990.tb02555.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  23 in total

1.  Isolation of three kinds of human endogenous retrovirus-like sequences using tRNA(Pro) as a probe.

Authors:  F Harada; N Tsukada; N Kato
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971

2.  Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus.

Authors:  P Sonigo; M Alizon; K Staskus; D Klatzmann; S Cole; O Danos; E Retzel; P Tiollais; A Haase; S Wain-Hobson
Journal:  Cell       Date:  1985-08       Impact factor: 41.582

3.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

4.  Nucleotide sequence of the haptoglobin and haptoglobin-related gene pair. The haptoglobin-related gene contains a retrovirus-like element.

Authors:  N Maeda
Journal:  J Biol Chem       Date:  1985-06-10       Impact factor: 5.157

5.  Envelope and long terminal repeat sequences of a cloned infectious NZB xenotropic murine leukemia virus.

Authors:  R R O'Neill; C E Buckler; T S Theodore; M A Martin; R Repaske
Journal:  J Virol       Date:  1985-01       Impact factor: 5.103

6.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

7.  Distinctive termini characterize two families of human endogenous retroviral sequences.

Authors:  P E Steele; A B Rabson; T Bryan; M A Martin
Journal:  Science       Date:  1984-08-31       Impact factor: 47.728

8.  Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease.

Authors:  M Yoshida; I Miyoshi; Y Hinuma
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Isolation of novel human retrovirus-related sequences by hybridization to synthetic oligonucleotides complementary to the tRNA(Pro) primer-binding site.

Authors:  B Kröger; I Horak
Journal:  J Virol       Date:  1987-07       Impact factor: 5.103
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  1 in total

1.  Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines.

Authors:  M Hirasawa; N Shijubo; T Uede; S Abe
Journal:  Br J Cancer       Date:  1994-09       Impact factor: 7.640
  1 in total

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