Literature DB >> 21125117

Construction of a thiamin sensor from the periplasmic thiamin binding protein.

Jeremiah W Hanes1, Debashree Chatterjee, Erika V Soriano, Steven E Ealick, Tadhg P Begley.   

Abstract

This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.

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Year:  2010        PMID： 21125117      PMCID： PMC3776480          DOI： 10.1039/c0cc04228e

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  8 in total

1.  Construction of a fluorescent biosensor family.

Authors:  Robert M de Lorimier; J Jeff Smith; Mary A Dwyer; Loren L Looger; Kevin M Sali; Chad D Paavola; Shahir S Rizk; Shamil Sadigov; David W Conrad; Leslie Loew; Homme W Hellinga
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

2.  An assay for thiaminase I in complex biological samples.

Authors:  Jeremiah W Hanes; Clifford E Kraft; Tadhg P Begley
Journal:  Anal Biochem       Date:  2007-06-07       Impact factor: 3.365

3.  Mystery of the poisoned expedition.

Authors:  J W Earl; B V McCleary
Journal:  Nature       Date:  1994-04-21       Impact factor: 49.962

4.  Vitamin B(1) status assessed by direct measurement of thiamin pyrophosphate in erythrocytes or whole blood by HPLC: comparison with erythrocyte transketolase activation assay.

Authors:  D Talwar; H Davidson; J Cooney; D St JO'Reilly
Journal:  Clin Chem       Date:  2000-05       Impact factor: 8.327

5.  Outbreak of life-threatening thiamine deficiency in infants in Israel caused by a defective soy-based formula.

Authors:  Aviva Fattal-Valevski; Anat Kesler; Ben-Ami Sela; Dorit Nitzan-Kaluski; Michael Rotstein; Ronit Mesterman; Hagit Toledano-Alhadef; Chaim Stolovitch; Chen Hoffmann; Omer Globus; Gideon Eshel
Journal:  Pediatrics       Date:  2005-02       Impact factor: 7.124

6.  Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase.

Authors:  M Brune; J L Hunter; J E Corrie; M R Webb
Journal:  Biochemistry       Date:  1994-07-12       Impact factor: 3.162

7.  Structural similarities between thiamin-binding protein and thiaminase-I suggest a common ancestor.

Authors:  Erika V Soriano; Kanagalaghatta R Rajashankar; Jeremiah W Hanes; Shridhar Bale; Tadhg P Begley; Steven E Ealick
Journal:  Biochemistry       Date:  2008-01-05       Impact factor: 3.162

8.  High prevalence of low plasma thiamine concentration in diabetes linked to a marker of vascular disease.

Authors:  P J Thornalley; R Babaei-Jadidi; H Al Ali; N Rabbani; A Antonysunil; J Larkin; A Ahmed; G Rayman; C W Bodmer
Journal:  Diabetologia       Date:  2007-08-04       Impact factor: 10.122
  8 in total
  3 in total

Review 1.  Thiamine Assays-Advances, Challenges, and Caveats.

Authors:  Katie A Edwards; Nicole Tu-Maung; Krystal Cheng; Binbin Wang; Antje J Baeumner; Clifford E Kraft
Journal:  ChemistryOpen       Date:  2017-03-08       Impact factor: 2.911

2.  Current Blockades of Proteins inside Nanopores for Real-Time Metabolome Analysis.

Authors:  Sarah Zernia; Nieck Jordy van der Heide; Nicole Stéphanie Galenkamp; Giorgos Gouridis; Giovanni Maglia
Journal:  ACS Nano       Date:  2020-02-07       Impact factor: 15.881

3.  Development of a Real-Time Pectic Oligosaccharide-Detecting Biosensor Using the Rapid and Flexible Computational Identification of Non-Disruptive Conjugation Sites (CINC) Biosensor Design Platform.

Authors:  Dustin D Smith; Joshua P King; D Wade Abbott; Hans-Joachim Wieden
Journal:  Sensors (Basel)       Date:  2022-01-26       Impact factor: 3.576
  3 in total

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