Literature DB >> 21123425

Microinjection of RNA and preparation of secreted proteins from Xenopus oocytes.

Hazel L Sive, Robert M Grainger, Richard M Harland.   

Abstract

Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) is a powerful technique for studying development, including protein expression during development. This protocol describes a method for the preparation of secreted (35)S-labeled proteins following mRNA microinjection into Xenopus oocytes.

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Year:  2010        PMID： 21123425     DOI： 10.1101/pdb.prot5538

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  4 in total

1.  Centriole biogenesis and function in multiciliated cells.

Authors:  Siwei Zhang; Brian J Mitchell
Journal:  Methods Cell Biol       Date:  2015-05-27       Impact factor: 1.441

2.  Patch-Clamp and Perfusion Techniques to Study Ion Channels Expressed in Xenopus Oocytes.

Authors:  Guohui Zhang; Jianmin Cui
Journal:  Cold Spring Harb Protoc       Date:  2018-04-02

3.  The Tudor-domain protein TDRD7, mutated in congenital cataract, controls the heat shock protein HSPB1 (HSP27) and lens fiber cell morphology.

Authors:  Carrie E Barnum; Salma Al Saai; Shaili D Patel; Catherine Cheng; Deepti Anand; Xiaolu Xu; Soma Dash; Archana D Siddam; Lisa Glazewski; Emily Paglione; Shawn W Polson; Shinichiro Chuma; Robert W Mason; Shuo Wei; Mona Batish; Velia M Fowler; Salil A Lachke
Journal:  Hum Mol Genet       Date:  2020-07-29       Impact factor: 6.150

4.  Tissue Rotation of the Xenopus Anterior-Posterior Neural Axis Reveals Profound but Transient Plasticity at the Mid-Gastrula Stage.

Authors:  Lyuba Bolkhovitinov; Bryan T Weselman; Gladys A Shaw; Chen Dong; Janhavi Giribhattanavar; Margaret S Saha
Journal:  J Dev Biol       Date:  2022-09-10
  4 in total

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