Literature DB >> 21123424

Calibration of the injection volume for microinjection of Xenopus oocytes and embryos.

Hazel L Sive, Robert M Grainger, Richard M Harland.   

Abstract

Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) or DNA is a powerful technique for studying development. Before microinjection can be performed, the injection volume must be calibrated carefully. This protocol describes the calibration procedure for a pressure injector.

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Year:  2010        PMID： 21123424     DOI： 10.1101/pdb.prot5537

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  6 in total

1.  Embryo microinjection of the lecithotrophic sea urchin Heliocidaris erythrogramma.

Authors:  Allison Edgar; Maria Byrne; Gregory A Wray
Journal:  J Biol Methods       Date:  2019-09-17

2.  ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia.

Authors:  Maximilian Haas; José Luis Gómez Vázquez; Dingyuan Iris Sun; Hong Thi Tran; Magdalena Brislinger; Alexia Tasca; Orr Shomroni; Kris Vleminckx; Peter Walentek
Journal:  Cell Rep       Date:  2019-09-24       Impact factor: 9.423

3.  Tissue Rotation of the Xenopus Anterior-Posterior Neural Axis Reveals Profound but Transient Plasticity at the Mid-Gastrula Stage.

Authors:  Lyuba Bolkhovitinov; Bryan T Weselman; Gladys A Shaw; Chen Dong; Janhavi Giribhattanavar; Margaret S Saha
Journal:  J Dev Biol       Date:  2022-09-10

4.  Controlled delivery of β-globin-targeting TALENs and CRISPR/Cas9 into mammalian cells for genome editing using microinjection.

Authors:  Renee N Cottle; Ciaran M Lee; David Archer; Gang Bao
Journal:  Sci Rep       Date:  2015-11-12       Impact factor: 4.379

5.  Eggshell ultrastructure and delivery of pharmacological inhibitors to the early embryo of R. prolixus by ethanol permeabilization of the extraembryonic layers.

Authors:  Larissa Bomfim; Priscila Vieira; Ariene Fonseca; Isabela Ramos
Journal:  PLoS One       Date:  2017-09-29       Impact factor: 3.240

6.  Drug Administration Routes Impact the Metabolism of a Synthetic Cannabinoid in the Zebrafish Larvae Model.

Authors:  Yu Mi Park; Markus R Meyer; Rolf Müller; Jennifer Herrmann
Journal:  Molecules       Date:  2020-09-29       Impact factor: 4.411
  6 in total

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