| Literature DB >> 21123060 |
Vinita Verma1, Sajan C Raju, Atya Kapley, Vipin Chandra Kalia, Gajanan S Kanade, Hatim F Daginawala, Hemant J Purohit.
Abstract
A strain, Stenotrophomonas HPC383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through HPLC and GC analysis. Culture HPC383 could degrade (%) various compounds (1 mM) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% crude oil. Gene locus dmpN, to identify phenol degrading capacity was determined by PCR followed by southern analysis. The sequenced DNA fragment exhibited 99% sequence similarity to phenol hydroxylase gene from Arthrobacter sp. W1 (FJ610336). Amino acid sequence analysis of phenol hydroxylase reveals it to belong to high-Ks (affinity constant) group. Application of HPC383 in bioremediation of aquatic and terrestrial sites contaminated with petrochemical has been suggested.Entities:
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Year: 2010 PMID： 21123060 DOI： 10.1016/j.biortech.2010.11.016
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642