Literature DB >> 2112286

Production and characterization of monoclonal antibodies specific for bovine gamma-interferon.

P R Wood1, J S Rothel, P G McWaters, S L Jones.   

Abstract

Nine stable hybridoma cell lines were established which secreted specific monoclonal antibodies (MAbs) to bovine gamma-interferon (BoIFN-gamma). Specific binding of each of the MAbs to recombinant BoIFN-gamma (rBoIFN-gamma) was demonstrated in an indirect ELISA, whilst none of the MAbs bound to rBoIFN-alpha or rBoIFN-beta. In a Western blot the MAbs reacted with the 16 kDa and 32 kDa polypeptides present in rBoIFN-gamma preparations. Competitive ELISA's showed that four MAbs bound to one epitope on rBoIFN-gamma, and the other five MAbs bound to a separate epitope. Two MAbs, each recognising different epitopes, were shown to neutralise the anti-viral activity of natural BoIFN-gamma.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2112286     DOI： 10.1016/0165-2427(90)90108-5

Source DB:  PubMed          Journal:  Vet Immunol Immunopathol        ISSN： 0165-2427            Impact factor:   2.046


  7 in total

1.  Systemic infusions of anti-interleukin-1β neutralizing antibodies reduce short-term brain injury after cerebral ischemia in the ovine fetus.

Authors:  Xiaodi Chen; Virginia Hovanesian; Syed Naqvi; Yow-Pin Lim; Richard Tucker; John E Donahue; Edward G Stopa; Barbara S Stonestreet
Journal:  Brain Behav Immun       Date:  2017-08-02       Impact factor: 7.217

2.  Anti-IL-6 neutralizing antibody modulates blood-brain barrier function in the ovine fetus.

Authors:  Jiyong Zhang; Grazyna B Sadowska; Xiaodi Chen; Seon Yeong Park; Jeong-Eun Kim; Courtney A Bodge; Erin Cummings; Yow-Pin Lim; Oleksandr Makeyev; Walter G Besio; John Gaitanis; William A Banks; Barbara S Stonestreet
Journal:  FASEB J       Date:  2015-01-21       Impact factor: 5.191

3.  In-vitro validation of cytokine neutralizing antibodies by testing with ovine mononuclear splenocytes.

Authors:  X Chen; S W Threlkeld; E E Cummings; G B Sadowska; Y-P Lim; J F Padbury; S Sharma; B S Stonestreet
Journal:  J Comp Pathol       Date:  2012-07-20       Impact factor: 1.311

4.  Expression of ovine gamma interferon in Escherichia coli and Corynebacterium glutamicum.

Authors:  H Billman-Jacobe; A L Hodgson; M Lightowlers; P R Wood; A J Radford
Journal:  Appl Environ Microbiol       Date:  1994-05       Impact factor: 4.792

5.  Development of an antibody to bovine IL-2 reveals multifunctional CD4 T(EM) cells in cattle naturally infected with bovine tuberculosis.

Authors:  Adam O Whelan; Bernardo Villarreal-Ramos; H Martin Vordermeier; Philip J Hogarth
Journal:  PLoS One       Date:  2011-12-22       Impact factor: 3.240

Review 6.  A review of the leishmanin skin test: A neglected test for a neglected disease.

Authors:  Jessica Carstens-Kass; Kayla Paulini; Patrick Lypaczewski; Greg Matlashewski
Journal:  PLoS Negl Trop Dis       Date:  2021-07-22

7.  Potential application of new diagnostic methods for controlling bovine tuberculosis in Brazil.

Authors:  Luciana Dos Santos Medeiros; Carla Dray Marassi; Eduardo Eustáquio S Figueiredo; Walter Lilenbaum
Journal:  Braz J Microbiol       Date:  2010-09-01       Impact factor: 2.476
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.