Cell-based cytokine therapy for skin rejuvenation.

The interaction between stem cells from human exfoliated deciduous teeth (SHEDs)-derived growth factors and human dermal fibroblast (HDF) has been investigated for the first time, as the application of SHEDs for dermal wound healing remains speculative. Stem cells from human exfoliated deciduous teeth have effects on HDFs by increasing collagen synthesis and by activating proliferation and migration activity of HDFs, suggesting that SHEDs or SHED-derived conditioned medium (SH-CM) can be used for the treatment of photoaging. Our results suggest that SHEDs and SH-CM should be constitutionally suited for photoaging treatment. Mainly with secreted growth factors or extracellular matrix proteins, SHEDs contribute to enhanced wound-healing potential of HDFs. Further mechanism studies using neutralizing antibodies against each growth factor may clarify the role of soluble factors of SHEDs in wound-healing process.