| Literature DB >> 21116970 |
Barry W Neun1, Stephan T Stern.
Abstract
Certain nanoparticles have been shown to accumulate within lysosome and hence may cause lysosomal pathologies such as phospholipidosis, lysosomal overload, and autophagy. This chapter describes a method for evaluation of lysosomal activity in porcine kidney cells (LLC-PK1) after exposure to nanoparticles. This method uses the accumulation of a cationic fluorescent dye (LysoTracker Red) in acidic cellular compartments as an indicator of total lysosome content. The lysotracker signal is normalized to the signal from a thiol-reactive dye which is proportional to the total number of viable cells.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21116970 DOI： 10.1007/978-1-60327-198-1_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745