Three-dimensional Flow Mapping in Microfiuidic Channels with Widefield Cross-correlation Microscopy.

Widefield cross-correlation microscopy enables large area measurements of flow vectors in microfluidic devices. Extending fluorescence cross-correlation spectroscopy, all points within each image give flow maps from image stacks acquired on a spinning-disk confocal microscope. The greater noise tolerance and efficient data collection for large volumes of interest enable even motion of single antibodies to be measured. A three-dimensional flow map for a typical microfluidic channel is evaluated, yielding full flow vector information. The method presented is general towards the input data, so applications concerning not only flow in microstructures, but biological transport and other microscopic motion phenomena are possible.