CONTEXT: Joint trauma is a risk factor for osteoarthritis (OA), which is becoming an increasingly important orthopedic concern for athletes and nonathletes alike. For advances in OA prevention, diagnosis, and treatment to occur, a greater understanding of the biochemical environment of the affected joint is needed. OBJECTIVE: To demonstrate the potential of a biochemical technique to enhance our understanding of and diagnostic capabilities for osteoarthritis. DESIGN: Cross-sectional. SETTING: Outpatient orthopedic practice. PARTICIPANTS: 8 subjects: 4 OA-knee participants (65 ± 6 y of age) and 4 normal-knee participants (54 ± 10 y) with no history of knee OA based on bilateral standing radiographs. INTERVENTION: The independent variable was group (OA knee, normal knee). MAIN OUTCOME MEASURES: 16 knee synovial-protein concentrations categorized as follows: 4 as pro-inflammatory, or catabolic, cytokines; 5 as anti-inflammatory, or protective, cytokines; 3 as catabolic enzymes; 2 as tissue inhibitors of metalloproteinases [TIMPs]; and 2 as adipokines. RESULTS: Two anti-inflammatory cytokines (interleukin [IL]-13 and osteoprotegerin) and a pro-inflammatory cytokine (IL-1β) were significantly lower in the OA knees. Two catabolic enzymes (matrix metalloproteinase [MMP]-2 and MMP-3) were significantly elevated in OA knees. TIMP-2, an inhibitor of MMPs, was significantly elevated in OA knees. CONCLUSIONS: Six of the 16 synovial-fluid proteins were significantly different between OA knees and normal knees in this study. Future research using a similar multiplex ELISA approach or other proteomic techniques may enable researchers and clinicians to develop more accurate biochemical profiles of synovial fluid to help diagnose OA, identify subsets of OA or individual characteristics, guide clinical decisions, and identify patients at risk for OA after knee injury.
CONTEXT: Joint trauma is a risk factor for osteoarthritis (OA), which is becoming an increasingly important orthopedic concern for athletes and nonathletes alike. For advances in OA prevention, diagnosis, and treatment to occur, a greater understanding of the biochemical environment of the affected joint is needed. OBJECTIVE: To demonstrate the potential of a biochemical technique to enhance our understanding of and diagnostic capabilities for osteoarthritis. DESIGN: Cross-sectional. SETTING:Outpatient orthopedic practice. PARTICIPANTS: 8 subjects: 4 OA-knee participants (65 ± 6 y of age) and 4 normal-knee participants (54 ± 10 y) with no history of knee OA based on bilateral standing radiographs. INTERVENTION: The independent variable was group (OA knee, normal knee). MAIN OUTCOME MEASURES: 16 knee synovial-protein concentrations categorized as follows: 4 as pro-inflammatory, or catabolic, cytokines; 5 as anti-inflammatory, or protective, cytokines; 3 as catabolic enzymes; 2 as tissue inhibitors of metalloproteinases [TIMPs]; and 2 as adipokines. RESULTS: Two anti-inflammatory cytokines (interleukin [IL]-13 and osteoprotegerin) and a pro-inflammatory cytokine (IL-1β) were significantly lower in the OA knees. Two catabolic enzymes (matrix metalloproteinase [MMP]-2 and MMP-3) were significantly elevated in OA knees. TIMP-2, an inhibitor of MMPs, was significantly elevated in OA knees. CONCLUSIONS: Six of the 16 synovial-fluid proteins were significantly different between OA knees and normal knees in this study. Future research using a similar multiplex ELISA approach or other proteomic techniques may enable researchers and clinicians to develop more accurate biochemical profiles of synovial fluid to help diagnose OA, identify subsets of OA or individual characteristics, guide clinical decisions, and identify patients at risk for OA after knee injury.
Authors: Thomas D Brown; Richard C Johnston; Charles L Saltzman; J Lawrence Marsh; Joseph A Buckwalter Journal: J Orthop Trauma Date: 2006 Nov-Dec Impact factor: 2.512
Authors: Peter R Kornaat; Johan L Bloem; Ruth Y T Ceulemans; Naghmeh Riyazi; Frits R Rosendaal; Rob G Nelissen; Wayne O Carter; Marie-Pierre Hellio Le Graverand; Margreet Kloppenburg Journal: Radiology Date: 2006-06 Impact factor: 11.105
Authors: Thomas Aigner; Katrin Fundel; Joachim Saas; Pia M Gebhard; Jochen Haag; Tilo Weiss; Alexander Zien; Franz Obermayr; Ralf Zimmer; Eckart Bartnik Journal: Arthritis Rheum Date: 2006-11
Authors: Reuben Gobezie; Alvin Kho; Bryan Krastins; David A Sarracino; Thomas S Thornhill; Michael Chase; Peter J Millett; David M Lee Journal: Arthritis Res Ther Date: 2007 Impact factor: 5.156
Authors: Nicole M Cattano; Jeffrey B Driban; Easwaran Balasubramanian; Mary F Barbe; Mamta Amin; Michael R Sitler Journal: BMC Musculoskelet Disord Date: 2011-11-28 Impact factor: 2.362
Authors: Olivier Bruyère; Cyrus Cooper; Nigel Arden; Jaime Branco; Maria Luisa Brandi; Gabriel Herrero-Beaumont; Francis Berenbaum; Elaine Dennison; Jean-Pierre Devogelaer; Marc Hochberg; John Kanis; Andrea Laslop; Tim McAlindon; Susanne Reiter; Pascal Richette; René Rizzoli; Jean-Yves Reginster Journal: Drugs Aging Date: 2015-03 Impact factor: 3.923