| Literature DB >> 21113173 |
Xin Gou1, Wei-Yang He, Ming-Zhao Xiao, Ming Qiu, Ming Wang, Yuan-Zhong Deng, Chao-Dong Liu, Zao-Bing Tang, Jie Li, Yong Chen.
Abstract
The present study investigated the effect of transplanting endothelial progenitor cells (EPCs) transfected with the vascular endothelial growth factor gene (VEGF165) into the corpora cavernosa of rats with diabetic erectile dysfunction (ED). A rat model of diabetic ED was constructed via intraperitoneal injection of streptozotocin. After streptozotocin treatment, pre-treated EPCs from each of three groups of rats were transplanted into their corpora cavernosa. Our results, following intracavernosal pressure (ICP) monitoring, showed that ICP increased significantly among rats in the trial group when compared to the results from rats in the blank-plasmid and control groups during basal conditions and electrical stimulation (P<0.01 for both comparisons). Histological examination revealed extensive neovascularisation in the corpora cavernosa of rats in the trial group. Fluorescence microscopy indicated that many of the transplanted EPCs in the trial group survived, differentiated into endothelial cells and integrated into the sites of neovascularisation. Based on the results of this study, we conclude that transplantation of VEGF165-transfected EPCs into the corpora cavernosa of rats with diabetic ED restores erectile function.Entities:
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Year: 2010 PMID： 21113173 PMCID： PMC3739184 DOI： 10.1038/aja.2010.116
Source DB: PubMed Journal: Asian J Androl ISSN： 1008-682X Impact factor: 3.285