| Literature DB >> 21111728 |
Gel R M Berardi1, Carmen K Rebelatto, Heloísa F Tavares, Max Ingberman, Patrícia Shigunov, Fabiane Barchiki, Alessandra M Aguiar, Nelson I Miyague, Julio C Francisco, Alejandro Correa, Alexandra C Senegaglia, Paula Hansen Suss, José A Moutinho, Vanessa S Sotomaior, Lia S Nakao, Paulo S Brofman.
Abstract
Stem cell therapy has been considered a promise for damaged myocardial tissue. We have previously shown that S-nitroso-N-acetyl-D,L-penicillamine (SNAP) increases the expression of several muscular markers and VEGF in mesenchymal stem cells, indicating that transplantation of SNAP-treated cells could provide better functional outcomes. Here, we transplanted SNAP-treated adipose tissue-derived stem cells (ADSCs) in rat infarcted myocardium. After 30days, we observed a significant improvement of the ejection fraction in rats that received SNAP-treated ADSCs, compared with those that received untreated cells (p=0.008). Immunohistochemical reactions showed an increased expression of troponin T-C and von Willebrand factor, and organized vascular units in the infarcted area of tissue transplanted with treated ADSCs. SNAP exposure induced intracellular S-nitrosation, a decreased GSH/GSSG ratio, but did not increase cGMP levels. Collectively, these results indicate that SNAP alters the redox environment of ADSCs, possibly associated with a pre-differentiation state, which may improve cardiac function after transplantation.Entities:
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Year: 2010 PMID： 21111728 DOI： 10.1016/j.yexmp.2010.11.005
Source DB: PubMed Journal: Exp Mol Pathol ISSN： 0014-4800 Impact factor: 3.362