Literature DB >> 21111698

A streamlined protocol for emulsion polymerase chain reaction and subsequent purification.

Tatjana Schütze1, Florian Rubelt, Julia Repkow, Nicole Greiner, Volker A Erdmann, Hans Lehrach, Zoltán Konthur, Jörn Glökler.   

Abstract

Compartmentalization of polymerase chain reaction (PCR) reduces artifacts, especially when complex libraries are amplified. It allows clonal amplification of templates from complex mixtures in a bias-free manner. Here we describe a rapid, straightforward, and easy protocol for PCR in a water-in-oil emulsion (ePCR) including sample recovery by DNA purification. Furthermore, no special laboratory equipment is needed and inexpensive components are used. Therefore, our flexible protocol allows ePCR to be readily implemented in daily routine experiments for a broad range of applications. 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 21111698     DOI： 10.1016/j.ab.2010.11.029

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  22 in total

1.  Diversity in the dynamical behaviour of a compartmentalized programmable biochemical oscillator.

Authors:  Maximilian Weitz; Jongmin Kim; Korbinian Kapsner; Erik Winfree; Elisa Franco; Friedrich C Simmel
Journal:  Nat Chem       Date:  2014-02-16       Impact factor: 24.427

Review 2.  Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.

Authors:  Zhanar Abil; Jared W Ellefson; Jimmy D Gollihar; Ella Watkins; Andrew D Ellington
Journal:  Nat Protoc       Date:  2017-11-09       Impact factor: 13.491

3.  Extensive libraries of gene truncation variants generated by in vitro transposition.

Authors:  Aleardo Morelli; Yari Cabezas; Lauren J Mills; Burckhard Seelig
Journal:  Nucleic Acids Res       Date:  2017-06-02       Impact factor: 16.971

4.  Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts.

Authors:  Ye Guan; Shiyuan Wang; Minjun Jin; Haotian Xu; Qiong Yang
Journal:  J Vis Exp       Date:  2018-09-27       Impact factor: 1.355

Review 5.  Functional assays for transcription mechanisms in high-throughput.

Authors:  Chenxi Qiu; Craig D Kaplan
Journal:  Methods       Date:  2019-02-20       Impact factor: 3.608

Review 6.  Aptamers as targeted therapeutics: current potential and challenges.

Authors:  Jiehua Zhou; John Rossi
Journal:  Nat Rev Drug Discov       Date:  2016-11-03       Impact factor: 84.694

7.  Interaction dynamics and virus-host range for estuarine actinophages captured by epicPCR.

Authors:  Eric G Sakowski; Keith Arora-Williams; Funing Tian; Ahmed A Zayed; Olivier Zablocki; Matthew B Sullivan; Sarah P Preheim
Journal:  Nat Microbiol       Date:  2021-02-25       Impact factor: 17.745

8.  Systematic dissection of regulatory motifs in 2000 predicted human enhancers using a massively parallel reporter assay.

Authors:  Pouya Kheradpour; Jason Ernst; Alexandre Melnikov; Peter Rogov; Li Wang; Xiaolan Zhang; Jessica Alston; Tarjei S Mikkelsen; Manolis Kellis
Journal:  Genome Res       Date:  2013-03-19       Impact factor: 9.043

9.  Enrichment of mutations in multiple DNA sequences using COLD-PCR in emulsion.

Authors:  Elena Castellanos-Rizaldos; Coren Audrey Milbury; G Mike Makrigiorgos
Journal:  PLoS One       Date:  2012-12-06       Impact factor: 3.240

10.  Onset of immune senescence defined by unbiased pyrosequencing of human immunoglobulin mRNA repertoires.

Authors:  Florian Rubelt; Volker Sievert; Florian Knaust; Christian Diener; Theam Soon Lim; Karl Skriner; Edda Klipp; Richard Reinhardt; Hans Lehrach; Zoltán Konthur
Journal:  PLoS One       Date:  2012-11-30       Impact factor: 3.240
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