Literature DB >> 211099

Immunoenzymehistochemical demonstration of the binding of low density lipoproteins to cultured human fibroblasts.

B J Vermeer, F C Reman, J J Emeis, C A De Haas-Van der Poel.   

Abstract

Using as indirect cytochemical immunoperoxidase technique, we were able to demonstrate the binding of low density lipoprotein to cultured human fibroblasts. With this technique, fibroblasts from a patient suffering from homozygous hyperlipoproteinaemia type IIa did not show this binding. The method described here allows study of the localization of unmodified low density lipoproteins binding to cultured fibroblasts.

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Year:  1978        PMID： 211099     DOI： 10.1007/bf00495980

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  12 in total

1.  Receptor-mediated control of cholesterol metabolism.

Authors:  M S Brown; J L Goldstein
Journal:  Science       Date:  1976-01-16       Impact factor: 47.728

2.  Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans.

Authors:  J L Goldstein; S K Basu; G Y Brunschede; M S Brown
Journal:  Cell       Date:  1976-01       Impact factor: 41.582

3.  Continuous pH-stat titration method for the assay of lipoprotein lipase activity in vitro.

Authors:  J Chung; A M Scanu
Journal:  Anal Biochem       Date:  1974-11       Impact factor: 3.365

4.  The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody.

Authors:  C R Taylor; J Burns
Journal:  J Clin Pathol       Date:  1974-01       Impact factor: 3.411

5.  Localization of lipase-like immunoreactivity in porcine adipose, aortic and myocardial tissue.

Authors:  W Nieuwenhuizen; J J Emeis; C M Van Sabben
Journal:  Atherosclerosis       Date:  1977-05       Impact factor: 5.162

6.  The immunohistochemical identification of hormone-producing cells in formalin-fixed, paraffin-embedded human pituitary tissue.

Authors:  A C Kruseman; G T Bots; E Lindeman
Journal:  J Pathol       Date:  1975-11       Impact factor: 7.996

7.  Binding, internalization, and degradation of low density lipoprotein by normal human fibroblasts and by fibroblasts from a case of homozygous familial hypercholesterolemia.

Authors:  O Stein; D B Weinstein; Y Stein; D Steinberg
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

8.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

9.  Metabolism of cationized lipoproteins by human fibroblasts. Biochemical and morphologic correlations.

Authors:  S K Basu; R G Anderson; J L Goldstein; M S Brown
Journal:  J Cell Biol       Date:  1977-07       Impact factor: 10.539

10.  Localization of low density lipoprotein receptors on plasma membrane of normal human fibroblasts and their absence in cells from a familial hypercholesterolemia homozygote.

Authors:  R G Anderson; J L Goldstein; M S Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205
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