| Literature DB >> 21107647 |
Hai-Xue Pan1, Ji-An Li, Ning-Jun He, Ji-Ye Chen, Yu-Meng Zhou, Lei Shao, Dai-Jie Chen.
Abstract
Spinosyns A and D are novel macrolide secondary metabolites containing two deoxysugars. Each of the sugars is derived from a common intermediate, which is biosynthesized sequentially by NDP-glucose synthase and glucose dehydratase. Systematical duplication of gtt and gdh was carried out by genetic engineering in Saccharopolyspora spinosa. Introduction of additional gtt and gdh genes under the control of PermE* promoter showed significant increase on their transcript levels. The production of spinosad was increased by over threefold to reach 800 μg/ml in the recombinant strain SIPI-M2093.Entities:
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Year: 2010 PMID： 21107647 DOI： 10.1007/s10529-010-0481-8
Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461