Stephen Hayes1, Sajad Ahmed, Peter Clark. 1. Department of Histopathology, Salford Royal NHS Foundation Trust, Salford, UK. stephen.hayes@srft.nhs.uk
Abstract
BACKGROUND: Barrett oesophagus (BO) occurs as a consequence of prolonged gastro-oesophageal reflux and it may precede the development of oesophageal adenocarcinoma. AIMS: The aim of this study was to accurately assess Cdx2 expression in the oesophageal metaplasia-dysplasia-adenocarcinoma sequence. METHOD: The expression of the transcription factor Cdx2 in Barrett metaplasia, high-grade glandular dysplasia and adenocarcinoma was investigated using immunohistochemistry. RESULTS: The results confirmed previous immunohistochemistry and PCR-based investigations, indicating that Cdx2 was expressed by intestinal metaplasia in the oesophagus. In addition, upregulation followed by linear downregulation of Cdx2 expression through the oesophageal metaplasia-dysplasia-adenocarcinoma sequence was demonstrated for the first time. CONCLUSION: Such downregulation of Cdx2 expression could be in keeping with a role as a tumour suppressor gene, but a simpler explanation would be downregulation of a differentiation marker.
BACKGROUND: Barrett oesophagus (BO) occurs as a consequence of prolonged gastro-oesophageal reflux and it may precede the development of oesophageal adenocarcinoma. AIMS: The aim of this study was to accurately assess Cdx2 expression in the oesophageal metaplasia-dysplasia-adenocarcinoma sequence. METHOD: The expression of the transcription factor Cdx2 in Barrett metaplasia, high-grade glandular dysplasia and adenocarcinoma was investigated using immunohistochemistry. RESULTS: The results confirmed previous immunohistochemistry and PCR-based investigations, indicating that Cdx2 was expressed by intestinal metaplasia in the oesophagus. In addition, upregulation followed by linear downregulation of Cdx2 expression through the oesophageal metaplasia-dysplasia-adenocarcinoma sequence was demonstrated for the first time. CONCLUSION: Such downregulation of Cdx2 expression could be in keeping with a role as a tumour suppressor gene, but a simpler explanation would be downregulation of a differentiation marker.
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