H Wang1, Z Yan, L Qiu, Z Hu, W Qian, L Xu. 1. Department of Cardiothoracic Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, People's Republic of China. wanght1974@hotmail.com
Abstract
OBJECTIVE: To evaluate the dynamic changes of PECAM-1/CD31 on pulmonary injury induced by ischemia-reperfusion (IR) in rats. METHODS: An isolated lung ischemia in rats was performed for 60 min, followed separately by 0, 2, 4, 6 or 8 h for reperfusion. At the end of reperfusion, the expression of PECAM-1/CD31 was detected by using flow cytometry (FCM). Simultaneously, we examined the ultrastructure changes of cells by transmission electron microscope. RESULTS: The expressions of PECAM-1/CD31 in neutrophils and lymphocytes of the blood were increased after IR; the peak was at 2 h after IR, then the expressions were gradually decreased to control level at 6 and 8 h after IR. Ultrastructural morphological changes including proliferation of alveolar type II and apoptotic bodies were seen after IR, the most prominently at 2 h after IR. CONCLUSION: PECAM-1/CD31 is involved in the pulmonary injury induced by IR.
OBJECTIVE: To evaluate the dynamic changes of PECAM-1/CD31 on pulmonary injury induced by ischemia-reperfusion (IR) in rats. METHODS: An isolated lung ischemia in rats was performed for 60 min, followed separately by 0, 2, 4, 6 or 8 h for reperfusion. At the end of reperfusion, the expression of PECAM-1/CD31 was detected by using flow cytometry (FCM). Simultaneously, we examined the ultrastructure changes of cells by transmission electron microscope. RESULTS: The expressions of PECAM-1/CD31 in neutrophils and lymphocytes of the blood were increased after IR; the peak was at 2 h after IR, then the expressions were gradually decreased to control level at 6 and 8 h after IR. Ultrastructural morphological changes including proliferation of alveolar type II and apoptotic bodies were seen after IR, the most prominently at 2 h after IR. CONCLUSION:PECAM-1/CD31 is involved in the pulmonary injury induced by IR.
Authors: D C Anderson; H S Glazer; J W Semenkovich; T K Pilgram; E P Trulock; J D Cooper; G A Patterson Journal: Radiology Date: 1995-04 Impact factor: 11.105
Authors: Guangju Ji; Christopher D O'Brien; Morris Feldman; Yefim Manevich; Poay Lim; Jing Sun; Steven M Albelda; Michael I Kotlikoff Journal: J Cell Biol Date: 2002-04-01 Impact factor: 10.539
Authors: David P Perrault; Gene K Lee; Antoun Bouz; Cynthia Sung; Roy Yu; Austin J Pourmoussa; Sun Young Park; Gene H Kim; Wan Jiao; Ketan M Patel; Young-Kwon Hong; Alex K Wong Journal: PLoS One Date: 2020-01-10 Impact factor: 3.240