Literature DB >> 21103565

Efficient and scalable serial extraction of DNA and RNA from frozen tissue samples.

Lucy Mathot1, Monica Lindman, Tobias Sjöblom.   

Abstract

Advances in cancer genomics have created a demand for scalable sample processing. We here present a process for serial extraction of nucleic acids from the same frozen tissue sample based on magnetic silica particles. The process is automation friendly with high recoveries of pure DNA and RNA suitable for analysis.

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Year:  2010        PMID: 21103565     DOI: 10.1039/c0cc02248a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  4 in total

1.  Superficial scrapings from breast tumors is a source for biobanking and research purposes.

Authors:  Ran Ma; Irma Fredriksson; Govindasamy-Muralidharan Karthik; Gregory Winn; Eva Darai-Ramqvist; Jonas Bergh; Johan Hartman
Journal:  Lab Invest       Date:  2014-04-28       Impact factor: 5.662

2.  Automated serial extraction of DNA and RNA from biobanked tissue specimens.

Authors:  Lucy Mathot; Monica Wallin; Tobias Sjöblom
Journal:  BMC Biotechnol       Date:  2013-08-19       Impact factor: 2.563

3.  RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter?

Authors:  Ali Akbar Samadani; Novin Nikbakhsh; Sadegh Fattahi; Roghayeh Pourbagher; Seyyed Mohsen Aghajanpour Mir; Narges Mousavi Kani; Zeinab Abedian; Haleh Akhavan-Niaki
Journal:  Int J Mol Cell Med       Date:  2015

4.  Automated genotyping of biobank samples by multiplex amplification of insertion/deletion polymorphisms.

Authors:  Lucy Mathot; Elin Falk-Sörqvist; Lotte Moens; Marie Allen; Tobias Sjöblom; Mats Nilsson
Journal:  PLoS One       Date:  2012-12-27       Impact factor: 3.240

  4 in total

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