High sensitivity and selectivity of human antibody attachment at the interstices between substrate-bound gold nanoparticles.

Durable Au-patterning nanostructures with uniform spacing having an average nanoparticle size of 8 nm and an interparticle gap of 11 nm were fabricated using a microwave-plasma oven. Single molecules of immunoglobulin G (IgG) are individually occupied at the space constraints so that the extinction response of localized surface plasmon resonance (LSPR) can be observed when detecting human anti-IgG molecules, affording a detection limit of 66.7 pM.