Literature DB >> 2110090

Rapid purification of the peptide toxins microcystin-LR and nodularin.

C Martin1, K Sivonen, U Matern, R Dierstein, J Weckesser.   

Abstract

An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammonium bicarbonate. An at least 95% purity was revealed on HPLC separation by monitoring at 214 nm. Application of the procedure to the cyclic pentapeptide toxin nodularin from Nodularia spumigena AV2 was examined.

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Year:  1990        PMID: 2110090     DOI: 10.1111/j.1574-6968.1990.tb04112.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  2 in total

1.  Isolation, purification, and characterization of newDaphnia-toxic compound from axenicMicrocystis flos-aquae strain PCC7806.

Authors:  D Jungmann
Journal:  J Chem Ecol       Date:  1995-11       Impact factor: 2.626

2.  Highly toxic Microcystis aeruginosa strain, isolated from São Paulo-Brazil, produce hepatotoxins and paralytic shellfish poison neurotoxins.

Authors:  Célia L Sant'Anna; Luciana R de Carvalho; Marli F Fiore; Maria Estela Silva-Stenico; Adriana S Lorenzi; Fernanda R Rios; Katsuhiro Konno; Carlos Garcia; Nestor Lagos
Journal:  Neurotox Res       Date:  2010-04-08       Impact factor: 3.911

  2 in total

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