OBJECTIVE: Ureaplasma respiratory tract colonization is associated with bronchopulmonary dysplasia (BPD) in preterm infants. Whether the 4 Ureaplasma parvum and 10 Ureaplasma urealyticum serovars differ in virulence is unknown. This study was conducted to determine the distribution of Ureaplasma serovars in respiratory secretions of a prospective cohort of preterm infants and to assess whether any of the serovars are associated with BPD. METHODS: Serial endotracheal and/or nasopharyngeal aspirates were obtained for Ureaplasma culture and PCR from 136 infants of gestational age <33 weeks. All positive samples were speciated and serovars were determined by real-time PCR. RESULTS: A total of 51 (37.5%) infants were Ureaplasma-positive one or more times during the first month of life. Respiratory colonization was inversely related to gestational age. Sixty-five percent of infants <26 weeks compared with 31% infants ≥ 26 weeks were culture or PCR positive. U. parvum was more common (N = 32, 63%) than U. urealyticum (N = 17, 33%); both species were present in 2 samples. Serovars 3 and 6 alone and in combination accounted for 96% U. parvum isolates. U. urealyticum isolates were commonly a mixture of multiple serovars, with serovar 11 alone or combined with other serovars (10/17, 59%) being the most common serovar. No individual species or serovars or serovar mixtures were associated with moderate-to-severe BPD. CONCLUSIONS: U. parvum serovars 3 and 6 and U. urealyticum serovar 11 were the most common serovars detected in respiratory samples from a prospective cohort of preterm infants.
OBJECTIVE:Ureaplasma respiratory tract colonization is associated with bronchopulmonary dysplasia (BPD) in preterm infants. Whether the 4 Ureaplasma parvum and 10 Ureaplasma urealyticum serovars differ in virulence is unknown. This study was conducted to determine the distribution of Ureaplasma serovars in respiratory secretions of a prospective cohort of preterm infants and to assess whether any of the serovars are associated with BPD. METHODS: Serial endotracheal and/or nasopharyngeal aspirates were obtained for Ureaplasma culture and PCR from 136 infants of gestational age <33 weeks. All positive samples were speciated and serovars were determined by real-time PCR. RESULTS: A total of 51 (37.5%) infants were Ureaplasma-positive one or more times during the first month of life. Respiratory colonization was inversely related to gestational age. Sixty-five percent of infants <26 weeks compared with 31% infants ≥ 26 weeks were culture or PCR positive. U. parvum was more common (N = 32, 63%) than U. urealyticum (N = 17, 33%); both species were present in 2 samples. Serovars 3 and 6 alone and in combination accounted for 96% U. parvum isolates. U. urealyticum isolates were commonly a mixture of multiple serovars, with serovar 11 alone or combined with other serovars (10/17, 59%) being the most common serovar. No individual species or serovars or serovar mixtures were associated with moderate-to-severe BPD. CONCLUSIONS:U. parvum serovars 3 and 6 and U. urealyticum serovar 11 were the most common serovars detected in respiratory samples from a prospective cohort of preterm infants.
Authors: A D Heggie; D Bar-Shain; B Boxerbaum; A A Fanaroff; M A O'Riordan; J A Robertson Journal: Pediatr Infect Dis J Date: 2001-09 Impact factor: 2.129
Authors: Li Xiao; John I Glass; Vanya Paralanov; Shibu Yooseph; Gail H Cassell; Lynn B Duffy; Ken B Waites Journal: J Clin Microbiol Date: 2010-06-16 Impact factor: 5.948
Authors: Rose M Viscardi; Catherine K Muhumuza; Andres Rodriguez; Karen D Fairchild; Chen-Chih J Sun; George W Gross; Andrew B Campbell; P David Wilson; Lisa Hester; Jeffrey D Hasday Journal: Pediatr Res Date: 2004-06 Impact factor: 3.756
Authors: Matthew S Payne; Kevin C W Goss; Gary J Connett; Julian P Legg; Ken D Bruce; Vicki Chalker Journal: J Clin Microbiol Date: 2011-12-21 Impact factor: 5.948
Authors: Ali F Aboklaish; Emilie Dordet-Frisoni; Christine Citti; Mark A Toleman; John I Glass; O Brad Spiller Journal: Int J Med Microbiol Date: 2014-09-26 Impact factor: 3.473
Authors: Matthew S Payne; Tania Tabone; Matthew W Kemp; Jeffrey A Keelan; O Brad Spiller; John P Newnham Journal: J Clin Microbiol Date: 2013-12-11 Impact factor: 5.948
Authors: Alexandra H Winters; Tricia D Levan; Stefanie N Vogel; Kirsty L Chesko; Toni I Pollin; Rose M Viscardi Journal: Pediatr Infect Dis J Date: 2013-08 Impact factor: 2.129