| Literature DB >> 2109718 |
P Brigidi1, E De Rossi, M L Bertarini, G Riccardi, D Matteuzzi.
Abstract
Plasmid DNAs were introduced by electroporation into Bacillus subtilis PB1424 as an alternative to competent-cell or protoplast transformation. The maximum electroporation efficiency was 10(4) transformants/microgram DNA. Parameters including growth phase of cells, ionic strength of the suspending medium, concentration and size of plasmid DNAs, amplitude and duration of the pulse, were evaluated in order to determine conditions that improved transformation efficiency.Entities:
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Year: 1990 PMID： 2109718 DOI： 10.1016/0378-1097(90)90182-p
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742