| Literature DB >> 2109612 |
T B Haugen1, E J Paulssen, J O Gordeladze, V Hansson.
Abstract
The 1.9 kb mRNA encoding the alpha subunit of Gs observed in the somatic cells of the rat testis was not seen in the germ cells (pachytene spermatocytes and round spermatids) as revealed by Northern blot analysis. Interestingly, the cDNA probe for Gs alpha detected a mRNA of shorter chain length (0.9 kb) exclusively in the haploid germ cells (round and elongated spermatids). However, no ADP ribosylated protein was detected in membranes from germ cells after cholera toxin treatment. The 0.9 kb transcript may represent a haploid germ cell-specific variant of Gs alpha, which is functionally different from the stimulatory component of the adenylate cyclase system.Entities:
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Year: 1990 PMID： 2109612 DOI： 10.1016/0006-291x(90)91679-m
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575