BACKGROUND: The AIN-93 diet was proposed by the American Institute of Nutrition with the objective of standardising studies in experimental nutrition. Our objective was to analyze the effects of AIN-93 diet after myocardial infarction in rats. METHODS: Post weaning, the animals were divided into two groups: control (C, n=62), fed the standard diet of our laboratory (Labina); AIN-93 Group (n=70), fed the AIN-93 diet. Achieving 250 g, the animals were subjected to myocardial infarction. RESULTS: Early mortality was increased in AIN-93 animals, associated with lower serum levels of calcium, magnesium, potassium, sodium, and phosphorus. On the other hand, after 90 days, AIN-93 showed smaller normalized left ventricular dimensions. The caloric and carbohydrate intake was smaller, but the fat intake was higher in AIN-93 rats. AIN-93 group also showed increased levels of β-hydroxyacylcoenzyme A dehydrogenase and citrate synthase. In addition, serum levels of insulin and cardiac levels of malondialdehyde, metalloproteinases-2 and -9, and TNF-α and IFN-γ were decreased in the AIN-93 group. CONCLUSION: AIN-93 diet increased early mortality, while attenuated the chronic remodeling process after experimental coronary occlusion. Therefore, this diet has biological effects and should be use with attention in this model.
BACKGROUND: The AIN-93 diet was proposed by the American Institute of Nutrition with the objective of standardising studies in experimental nutrition. Our objective was to analyze the effects of AIN-93 diet after myocardial infarction in rats. METHODS: Post weaning, the animals were divided into two groups: control (C, n=62), fed the standard diet of our laboratory (Labina); AIN-93 Group (n=70), fed the AIN-93 diet. Achieving 250 g, the animals were subjected to myocardial infarction. RESULTS: Early mortality was increased in AIN-93 animals, associated with lower serum levels of calcium, magnesium, potassium, sodium, and phosphorus. On the other hand, after 90 days, AIN-93 showed smaller normalized left ventricular dimensions. The caloric and carbohydrate intake was smaller, but the fat intake was higher in AIN-93rats. AIN-93 group also showed increased levels of β-hydroxyacylcoenzyme A dehydrogenase and citrate synthase. In addition, serum levels of insulin and cardiac levels of malondialdehyde, metalloproteinases-2 and -9, and TNF-α and IFN-γ were decreased in the AIN-93 group. CONCLUSION:AIN-93 diet increased early mortality, while attenuated the chronic remodeling process after experimental coronary occlusion. Therefore, this diet has biological effects and should be use with attention in this model.
Authors: Priscila Portugal Dos Santos; Bruna Paola Murino Rafacho; Andréa de Freitas Gonçalves; Rodrigo Gibin Jaldin; Thiago Bruder do Nascimento; Marcondes Alves Barbosa Silva; Stêfany Bruno Assis Cau; Meliza Goi Roscani; Paula Schimdt Azevedo; Marcos Ferreira Minicucci; Rita de Cássia Tostes; Leonardo Antonio Memede Zornoff; Sergio Alberto Rupp de Paiva Journal: PLoS One Date: 2014-06-12 Impact factor: 3.240
Authors: Marcos F Minicucci; Priscila P dos Santos; Bruna P M Rafacho; Andrea F Gonçalves; Renata A C Silva; Fernanda Chiuso-Minicucci; Paula S Azevedo; Bertha F Polegato; Katashi Okoshi; Elenize J Pereira; Sergio A R Paiva; Leonardo A M Zornoff Journal: PLoS One Date: 2013-09-30 Impact factor: 3.240