PURPOSE: to test whether a single episode of early-life seizures may interfere with the development of the cerebellum. The cerebellum is particularly vulnerable in infants, since it is characterized by an important postnatal histogenesis that leads to the settling of adult circuitry. METHODS: seizures were induced in 10-day-old Wistar rats with a single convulsive dose (80μg/g b.w., s.c.) of pentylentetrazole (PTZ). Immediately after rats were treated with (3)H-thymidine ((3)HTdR, 2.5μCi/g b.w, s.c.). Rats were killed 4h later and paraffin sections of the cerebellar vermis were processed for (3)HTdR autoradiography and immunocytochemistry for 2/3 subunits of AMPA glutamate receptor (GluR2/3), glutamate transporter 1 (GLT1) and calbindin. RESULTS: seizures reduced the proliferation rate of cells in the external germinal layer. Purkinje cells showed increased GluR2/3 immunoreactivity. However, some Purkinje cells were unstained or lost. Increased GLT1 immunoreactivity was present in glial cells surrounding Purkinje cells. Calbindin immunoreaction confirmed that some Purkinje cells were missed. The remaining Purkinje cells showed large spheroids along the course of their axon. CONCLUSIONS: data indicate that seizures lead to a loss and alteration of Purkinje cells in the cerebellum of immature rats. Since at 10 days of life Purkinje cells are no more proliferating, the loss of Purkinje cells should be permanent. 2010 Elsevier B.V. All rights reserved.
PURPOSE: to test whether a single episode of early-life seizures may interfere with the development of the cerebellum. The cerebellum is particularly vulnerable in infants, since it is characterized by an important postnatal histogenesis that leads to the settling of adult circuitry. METHODS:seizures were induced in 10-day-old Wistar rats with a single convulsive dose (80μg/g b.w., s.c.) of pentylentetrazole (PTZ). Immediately after rats were treated with (3)H-thymidine ((3)HTdR, 2.5μCi/g b.w, s.c.). Rats were killed 4h later and paraffin sections of the cerebellar vermis were processed for (3)HTdR autoradiography and immunocytochemistry for 2/3 subunits of AMPA glutamate receptor (GluR2/3), glutamate transporter 1 (GLT1) and calbindin. RESULTS:seizures reduced the proliferation rate of cells in the external germinal layer. Purkinje cells showed increased GluR2/3 immunoreactivity. However, some Purkinje cells were unstained or lost. Increased GLT1 immunoreactivity was present in glial cells surrounding Purkinje cells. Calbindin immunoreaction confirmed that some Purkinje cells were missed. The remaining Purkinje cells showed large spheroids along the course of their axon. CONCLUSIONS: data indicate that seizures lead to a loss and alteration of Purkinje cells in the cerebellum of immature rats. Since at 10 days of life Purkinje cells are no more proliferating, the loss of Purkinje cells should be permanent. 2010 Elsevier B.V. All rights reserved.
Authors: Kaspar Matiasek; Martí Pumarola I Batlle; Marco Rosati; Francisco Fernández-Flores; Andrea Fischer; Eva Wagner; Mette Berendt; Sofie F M Bhatti; Luisa De Risio; Robyn G Farquhar; Sam Long; Karen Muñana; Edward E Patterson; Akos Pakozdy; Jacques Penderis; Simon Platt; Michael Podell; Heidrun Potschka; Clare Rusbridge; Veronika M Stein; Andrea Tipold; Holger A Volk Journal: BMC Vet Res Date: 2015-08-28 Impact factor: 2.741
Authors: Anna Khimchenko; Christos Bikis; Alexandra Pacureanu; Simone E Hieber; Peter Thalmann; Hans Deyhle; Gabriel Schweighauser; Jürgen Hench; Stephan Frank; Magdalena Müller-Gerbl; Georg Schulz; Peter Cloetens; Bert Müller Journal: Adv Sci (Weinh) Date: 2018-03-30 Impact factor: 16.806