OBJECTIVES: The study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions. METHODS: A total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA. RESULTS: Malignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d. CONCLUSIONS: Cell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.
OBJECTIVES: The study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions. METHODS: A total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA. RESULTS:Malignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d. CONCLUSIONS: Cell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.
Authors: Yoon Mi Shin; Jieun Yun; Ok-Jun Lee; Hye-Suk Han; Sung-Nam Lim; Jin Young An; Ki Hyeong Lee; Ki Man Lee; Kang Hyeon Choe Journal: Cancer Res Treat Date: 2014-04-22 Impact factor: 4.679
Authors: Shengnan Wei; Ming Zhang; Yang Yu; Xiaoxin Lan; Fan Yao; Xin Yan; Li Chen; Grant M Hatch Journal: PLoS One Date: 2016-03-24 Impact factor: 3.240