OBJECTIVES: To gain information about overexpressed antigens in renal cell carcinoma (RCC) by using a chemical proteomics approach. METHODS: RCC cell line 769P was cultured and proteome analysis was subsequently carried out in the culture supernatants. By using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and tandem mass spectrometry (LC-MS/MS), proteins in the culture supernatants were searched. A MEDLINE search to define the functions of the identified proteins was carried out. RESULTS: Four differentially regulated proteins (profilin 1, amyloid beta A4 protein [APP], proprotein convertase subtilisin/kexin type 1 inhibitor [ProSAAS], galectin-3-binding protein [LGALS3BP]) were selected. These were not overexpressed in normal kidney tissue or reported in RCC. Their levels were measured through western blotting of normal kidney and RCC tissues. No differences were observed in the expression levels of APP, ProSAAS or LGALS3BP between RCC and normal kidney tissues. Profilin 1 was overexpressed in RCC tissue. On the basis of this observation, an immunohistochemical analysis of profilin 1 in normal kidney and RCC tissues was carried out. In normal tissues, tubules that were sources of RCC stained positive for profilin 1. In RCC tissue, in contrast, the stromal cells in the tumors stained positive. CONCLUSIONS: Profilin 1 can be a key element in the pathological processes of RCC, such as tumorigenesis and/or tumor growth. Thus, it has the potential to serve as a diagnostic or progression biomarker and therapeutic target in RCC.
OBJECTIVES: To gain information about overexpressed antigens in renal cell carcinoma (RCC) by using a chemical proteomics approach. METHODS:RCC cell line 769P was cultured and proteome analysis was subsequently carried out in the culture supernatants. By using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and tandem mass spectrometry (LC-MS/MS), proteins in the culture supernatants were searched. A MEDLINE search to define the functions of the identified proteins was carried out. RESULTS: Four differentially regulated proteins (profilin 1, amyloid beta A4 protein [APP], proprotein convertase subtilisin/kexin type 1 inhibitor [ProSAAS], galectin-3-binding protein [LGALS3BP]) were selected. These were not overexpressed in normal kidney tissue or reported in RCC. Their levels were measured through western blotting of normal kidney and RCC tissues. No differences were observed in the expression levels of APP, ProSAAS or LGALS3BP between RCC and normal kidney tissues. Profilin 1 was overexpressed in RCC tissue. On the basis of this observation, an immunohistochemical analysis of profilin 1 in normal kidney and RCC tissues was carried out. In normal tissues, tubules that were sources of RCC stained positive for profilin 1. In RCC tissue, in contrast, the stromal cells in the tumors stained positive. CONCLUSIONS:Profilin 1 can be a key element in the pathological processes of RCC, such as tumorigenesis and/or tumor growth. Thus, it has the potential to serve as a diagnostic or progression biomarker and therapeutic target in RCC.
Authors: Abigail Allen; David Gau; Paul Francoeur; Jordan Sturm; Yue Wang; Ryan Martin; Jodi Maranchie; Anette Duensing; Adam Kaczorowski; Stefan Duensing; Lily Wu; Michael T Lotze; David Koes; Walter J Storkus; Partha Roy Journal: J Biol Chem Date: 2020-09-03 Impact factor: 5.157
Authors: Xue-Ling Kang; Hong Zou; Li Juan Pang; Wen Hao Hu; Jin Zhao; Yan Qi; Chun-Xia Liu; Jian Ming Hu; Jing-Xia Tang; Hong An Li; Wei Hua Liang; Xiang-Lin Yuan; Feng Li Journal: Int J Clin Exp Pathol Date: 2015-04-01
Authors: Toni K Choueiri; Laurence Albiges; Michael B Atkins; Ziad Bakouny; Gennady Bratslavsky; David A Braun; Naomi B Haas; John B A G Haanen; A Ari Hakimi; Michael A S Jewett; Eric Jonasch; William G Kaelin; Payal Kapur; Chris Labaki; Bryan Lewis; David F McDermott; Sumanta K Pal; Kevin Pels; Susan Poteat; Thomas Powles; W Kimryn Rathmell; Brian I Rini; Sabina Signoretti; Nizar M Tannir; Robert G Uzzo; Hans J Hammers Journal: Clin Cancer Res Date: 2022-03-01 Impact factor: 13.801
Authors: Olena Masui; Nicole M A White; Leroi V DeSouza; Olga Krakovska; Ajay Matta; Shereen Metias; Bishoy Khalil; Alexander D Romaschin; R John Honey; Robert Stewart; Kenneth Pace; Georg A Bjarnason; K W Michael Siu; George M Yousef Journal: Mol Cell Proteomics Date: 2012-10-17 Impact factor: 5.911