PURPOSE: To analyze the distribution of tarsal elastic fibers, with special emphasis on the meibomian duct area. METHODS: Nine postmortem orbits (5 right, 4 left) of 7 Asian cadavers (mean age at death: 82 years, age range: 73-94 years), fixed in 10% buffered formalin, were used. Full-thickness sagittal sections of the upper eyelid specimens were dehydrated and embedded in paraffin and cut in 7-μm-thick sagittal sections. Sliced sections were stained with elastica van Gieson. RESULTS: Elastic fibers were demonstrated in all tarsal plates. They were mostly distributed around the meibomian ducts and much less around the acini or in the tarsal stroma. These elastic fibers were also seen in the area of the muscle of Riolan. The outer part of the meibomian acini was less surrounded by elastic fibers. CONCLUSIONS: Tarsal elastic fibers are distributed mainly around the meibomian ducts, and much less around the acini or in the tarsal stroma. The fibers were also shown to extend to the area of the muscle of Riolan. These anatomical findings may help clarify the mechanisms involved in meibomian secretion.
PURPOSE: To analyze the distribution of tarsal elastic fibers, with special emphasis on the meibomian duct area. METHODS: Nine postmortem orbits (5 right, 4 left) of 7 Asian cadavers (mean age at death: 82 years, age range: 73-94 years), fixed in 10% buffered formalin, were used. Full-thickness sagittal sections of the upper eyelid specimens were dehydrated and embedded in paraffin and cut in 7-μm-thick sagittal sections. Sliced sections were stained with elastica van Gieson. RESULTS: Elastic fibers were demonstrated in all tarsal plates. They were mostly distributed around the meibomian ducts and much less around the acini or in the tarsal stroma. These elastic fibers were also seen in the area of the muscle of Riolan. The outer part of the meibomian acini was less surrounded by elastic fibers. CONCLUSIONS: Tarsal elastic fibers are distributed mainly around the meibomian ducts, and much less around the acini or in the tarsal stroma. The fibers were also shown to extend to the area of the muscle of Riolan. These anatomical findings may help clarify the mechanisms involved in meibomian secretion.