OBJECTIVE: To better define the nature of the transient neutropenia shortly following granulocyte-colony stimulating factor (G-CSF) administration. MATERIALS AND METHODS: To evaluate the disappearance of neutrophils, we investigated neutrophil trafficking. Ratios of neutrophil number to background cellularity for C57BL/6 LysM-EGFP knock-in mice and rhesus macaques were determined in the lung, liver, spleen, and kidney after G-CSF administration. RESULTS: For the C57BL/6 LysM-EGFP knock-in mice, the enhanced green fluorescent protein expression (EGFP(+)) cells increased in the lung and spleen within 15 minutes of administering 50 μg/kg G-CSF subcutaneously, and continued to increase in the lung and spleen from 15 minutes to 30 minutes. At 240 minutes, the pulmonary infiltrate declined to a level comparable to the level at 15 minutes, while in the spleen EGFP(+) cells continued to increase. For rhesus macaques, CD18(+) cells also significantly increased in the lung 30 minutes after administration of 10 μg/kg G-CSF subcutaneously compared to the control level. CONCLUSIONS: These results suggest that the transient neutropenia following G-CSF administration in the mouse and nonhuman primate is associated with an accumulation of neutrophils within pulmonary and splenic vasculature. Published by Elsevier Inc.
OBJECTIVE: To better define the nature of the transient neutropenia shortly following granulocyte-colony stimulating factor (G-CSF) administration. MATERIALS AND METHODS: To evaluate the disappearance of neutrophils, we investigated neutrophil trafficking. Ratios of neutrophil number to background cellularity for C57BL/6 LysM-EGFP knock-in mice and rhesus macaques were determined in the lung, liver, spleen, and kidney after G-CSF administration. RESULTS: For the C57BL/6 LysM-EGFP knock-in mice, the enhanced green fluorescent protein expression (EGFP(+)) cells increased in the lung and spleen within 15 minutes of administering 50 μg/kg G-CSF subcutaneously, and continued to increase in the lung and spleen from 15 minutes to 30 minutes. At 240 minutes, the pulmonary infiltrate declined to a level comparable to the level at 15 minutes, while in the spleen EGFP(+) cells continued to increase. For rhesus macaques, CD18(+) cells also significantly increased in the lung 30 minutes after administration of 10 μg/kg G-CSF subcutaneously compared to the control level. CONCLUSIONS: These results suggest that the transient neutropenia following G-CSF administration in the mouse and nonhuman primate is associated with an accumulation of neutrophils within pulmonary and splenic vasculature. Published by Elsevier Inc.
Authors: A Falanga; M Marchetti; V Evangelista; S Manarini; E Oldani; S Giovanelli; M Galbusera; C Cerletti; T Barbui Journal: Blood Date: 1999-04-15 Impact factor: 22.113
Authors: Juan T Borda; Xavier Alvarez; Mahesh Mohan; Atsuhiko Hasegawa; Andrea Bernardino; Sherrie Jean; Pyone Aye; Andrew A Lackner Journal: Am J Pathol Date: 2008-02-14 Impact factor: 4.307
Authors: Brent C Gordon; Amy M Revenis; Aylin C Bonifacino; William E Sander; Mark E Metzger; Allen E Krouse; Tatiana N Usherson; Robert E Donahue Journal: Exp Hematol Date: 2007-06 Impact factor: 3.084
Authors: M de Haas; J M Kerst; C E van der Schoot; J Calafat; C E Hack; J H Nuijens; D Roos; R H van Oers; A E von dem Borne Journal: Blood Date: 1994-12-01 Impact factor: 22.113
Authors: Robert E Donahue; Laura Tuschong; Thomas R Bauer; Yu Ying Yau; Susan F Leitman; Dennis D Hickstein Journal: Blood Date: 2011-08-15 Impact factor: 22.113
Authors: Smriti Mehra; Xavier Alvarez; Peter J Didier; Lara A Doyle; James L Blanchard; Andrew A Lackner; Deepak Kaushal Journal: J Infect Dis Date: 2012-12-18 Impact factor: 5.226