Literature DB >> 21087639

Quantitation of ranaviruses in cell culture and tissue samples.

Riikka Holopainen1, Jarno Honkanen, Britt Bang Jensen, Ellen Ariel, Hannele Tapiovaara.   

Abstract

A quantitative real-time PCR (qPCR) based on a standard curve was developed for detection and quantitation of ranaviruses. The target gene for the qPCR was viral DNA polymerase (DNApol). All ten ranavirus isolates studied (Epizootic haematopoietic necrosis virus, EHNV; European catfish virus, ECV; European sheatfish virus, ESV; Frog virus 3, FV3; Bohle iridovirus, BIV; Doctor fish virus, DFV; Guppy virus 6, GV6; Pike-perch iridovirus, PPIV; Rana esculenta virus Italy 282/I02, REV282/I02 and Short-finned eel ranavirus, SERV) were detected with the qPCR assay. In addition, two fish cell lines - epithelioma papulosum cyprini (EPC) and bluegill fry (BF-2) - were infected with four of the isolates (EHNV, ECV, FV3 and DFV), and the viral quantity was determined from seven time points during the first three days after infection. The qPCR was also used to determine the viral load in tissue samples from pike (Esox lucius) fry challenged experimentally with EHNV.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 21087639     DOI： 10.1016/j.jviromet.2010.11.004

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  7 in total

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Journal:  Virol J       Date:  2013-11-23       Impact factor: 4.099

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Journal:  Emerg Microbes Infect       Date:  2018-04-04       Impact factor: 7.163

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Authors:  Sang Wha Kim; Sib Sankar Giri; Sang Guen Kim; Jun Kwon; Woo Taek Oh; Se Chang Park
Journal:  Pathogens       Date:  2020-03-17

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  7 in total

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