BACKGROUND: An increase in interleukin (IL)-18 production from epidermal cells has been reported in an atopic dermatitis (AD) mouse model, and subsequent topical application of Staphylococcus aureus results in severe dermatitis. OBJECTIVES: To reveal the relationship between S. aureus colonization of skin lesions and keratinocyte IL-18 production, particularly in AD with relatively low serum IgE levels. We also aimed to establish a simple and noninvasive method of assaying IL-18 produced by epidermal keratinocytes to evaluate local skin inflammation and therapeutic effects in patients with AD. METHODS: IL-18 in the horny layer of the skin was collected via a tape-stripping method and measured in 95 patients with AD and 40 healthy controls by enzyme-linked immunosorbent assay (ELISA). Clinical severity, blood data and S. aureus skin colonization were evaluated before and after treatment. RESULTS: IL-18 levels in the horny layer were significantly higher in the skin lesions of patients with AD than in healthy controls and correlated with SCORAD, levels of serum IL-18, IgE, lactate dehydrogenase, thymus and activation-regulated chemokine, blood eosinophils and transepidermal water loss. In the AD group with serum IgE < 1500 IU mL(-1) , significantly higher IL-18 levels were observed in the horny layer of patients colonized with S. aureus compared with those who were not. CONCLUSIONS: Epidermal IL-18 production was associated with the severity of AD. Staphylococcus aureus colonization seems to contribute to this IL-18 production, especially in the AD group with relatively low IgE production. Tape stripping provides an easy and noninvasive method to assess epidermal IL-18 production by ELISA.
BACKGROUND: An increase in interleukin (IL)-18 production from epidermal cells has been reported in an atopic dermatitis (AD) mouse model, and subsequent topical application of Staphylococcus aureus results in severe dermatitis. OBJECTIVES: To reveal the relationship between S. aureus colonization of skin lesions and keratinocyte IL-18 production, particularly in AD with relatively low serum IgE levels. We also aimed to establish a simple and noninvasive method of assaying IL-18 produced by epidermal keratinocytes to evaluate local skin inflammation and therapeutic effects in patients with AD. METHODS:IL-18 in the horny layer of the skin was collected via a tape-stripping method and measured in 95 patients with AD and 40 healthy controls by enzyme-linked immunosorbent assay (ELISA). Clinical severity, blood data and S. aureus skin colonization were evaluated before and after treatment. RESULTS:IL-18 levels in the horny layer were significantly higher in the skin lesions of patients with AD than in healthy controls and correlated with SCORAD, levels of serum IL-18, IgE, lactate dehydrogenase, thymus and activation-regulated chemokine, blood eosinophils and transepidermal water loss. In the AD group with serum IgE < 1500 IU mL(-1) , significantly higher IL-18 levels were observed in the horny layer of patients colonized with S. aureus compared with those who were not. CONCLUSIONS: Epidermal IL-18 production was associated with the severity of AD. Staphylococcus aureus colonization seems to contribute to this IL-18 production, especially in the AD group with relatively low IgE production. Tape stripping provides an easy and noninvasive method to assess epidermal IL-18 production by ELISA.
Authors: Y Sano; K Masuda; R Tamagawa-Mineoka; H Matsunaka; Y Murakami; R Yamashita; E Morita; N Katoh Journal: Clin Exp Immunol Date: 2013-03 Impact factor: 4.330
Authors: Haiyun Liu; Nathan K Archer; Carly A Dillen; Yu Wang; Alyssa G Ashbaugh; Roger V Ortines; Tracy Kao; Steven K Lee; Shuting S Cai; Robert J Miller; Mark C Marchitto; Emily Zhang; Daniel P Riggins; Roger D Plaut; Scott Stibitz; Raif S Geha; Lloyd S Miller Journal: Cell Host Microbe Date: 2017-11-08 Impact factor: 21.023
Authors: Sanja Kezic; Gráinne M O'Regan; René Lutter; Ivone Jakasa; Ellen S Koster; Sean Saunders; Peter Caspers; Patrick M J H Kemperman; Gerwin J Puppels; Aileen Sandilands; Huijia Chen; Linda E Campbell; Karin Kroboth; Rosemarie Watson; Padraic G Fallon; W H Irwin McLean; Alan D Irvine Journal: J Allergy Clin Immunol Date: 2012-02-08 Impact factor: 10.793
Authors: M A McAleer; I Jakasa; G Hurault; P Sarvari; W H I McLean; R J Tanaka; S Kezic; A D Irvine Journal: Br J Dermatol Date: 2018-10-04 Impact factor: 9.302