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Literature DB >> 2108430

Circular chromosomal DNA in the sulfur-dependent archaebacterium Sulfolobus acidocaldarius.

Abstract

The shape of the chromosomal DNA of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius was analyzed by the pulsed-field gel electrophoresis(PFGE). S.acidocaldarius DNA digested with Notl showed two DNA bands at around 1.0 Mbp and 2.1 Mbp. Notl-linking clones were isolated from the library of S.acidocaldarius chromosomal DNA. It contained two Notl sites. Both 1.0 and 2.1 Mbp DNA band separated by PFGE were hybridized with the two independent Notl-linking fragment. Each right and left arms of two Notl-linking fragments were hybridized with one of the two DNA bands separated by PFGE. The results indicated that the chromosomal DNA of S.acidocaldarius is circular.

Entities: Chemical Species

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Year: 1990 PMID： 2108430 PMCID： PMC330426 DOI： 10.1093/nar/18.5.1133

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

6. Molecular characterization of a thermostable aldehyde dehydrogenase (ALDH) from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7.

Authors: Tianming Liu; Lujiang Hao; Ruiming Wang; Bo Liu
Journal: Extremophiles Date: 2012-12-06 Impact factor: 2.395

6 in total

Circular chromosomal DNA in the sulfur-dependent archaebacterium Sulfolobus acidocaldarius.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Size and physical map of the chromosome of Haemophilus influenzae.

3. Phylogenetic structure of the prokaryotic domain: the primary kingdoms.

4. DNA organization of Methanobacterium thermoautotrophicum.

5. Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature.

6. Circularity of the Bacillus subtilis chromosome and further studies on its bidirectional replication.

7. Origin of concatemeric T7 DNA.

8. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

9. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

10. Size and structure of the Mycoplasma hominis H39 chromosome.

1. The first crystal structure of an archaeal helical repeat protein.

2. A physical map of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius 7 chromosome.

3. Molecular biology of extremophiles.

4. Using protein synthesis inhibitors to establish the phylogenetic relationships of the Sulfolobales order.

Review 5. Organization of the bacterial chromosome.

6. Molecular characterization of a thermostable aldehyde dehydrogenase (ALDH) from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7.