BACKGROUND: Artemether-lumefantrine is the most widely recommended artemisinin-based combination treatment for falciparum malaria. Quantification of artemether and its metabolite dihydroartemisinin in biological matrices has traditionally been difficult, with sensitivity being an issue. RESULTS: A high-throughput bioanalytical method for the analysis of artemether and its metabolite dihydroartemisinin in human plasma using solid-phase extraction in the 96-well plate format and liquid chromatography coupled to positive ion mode tandem mass spectroscopy has been developed and validated according to US FDA guidelines. The method uses 50 µl plasma and covers the calibration range 1.43-500 ng/ml with a limit of detection at 0.36 ng/ml. CONCLUSIONS: The developed liquid chromatography-tandem mass spectrometry assay is more sensitive than all previous methods despite using a lower plasma volume (50 µl) and is highly suitable for clinical studies where plasma volumes are limited, such as pediatric trials.
BACKGROUND:Artemether-lumefantrine is the most widely recommended artemisinin-based combination treatment for falciparum malaria. Quantification of artemether and its metabolite dihydroartemisinin in biological matrices has traditionally been difficult, with sensitivity being an issue. RESULTS: A high-throughput bioanalytical method for the analysis of artemether and its metabolite dihydroartemisinin in human plasma using solid-phase extraction in the 96-well plate format and liquid chromatography coupled to positive ion mode tandem mass spectroscopy has been developed and validated according to US FDA guidelines. The method uses 50 µl plasma and covers the calibration range 1.43-500 ng/ml with a limit of detection at 0.36 ng/ml. CONCLUSIONS: The developed liquid chromatography-tandem mass spectrometry assay is more sensitive than all previous methods despite using a lower plasma volume (50 µl) and is highly suitable for clinical studies where plasma volumes are limited, such as pediatric trials.
Authors: Rob W van der Pluijm; Rupam Tripura; Richard M Hoglund; Aung Pyae Phyo; Dysoley Lek; Akhter Ul Islam; Anupkumar R Anvikar; Parthasarathi Satpathi; Sanghamitra Satpathi; Prativa Kumari Behera; Amar Tripura; Subrata Baidya; Marie Onyamboko; Nguyen Hoang Chau; Yok Sovann; Seila Suon; Sokunthea Sreng; Sivanna Mao; Savuth Oun; Sovannary Yen; Chanaki Amaratunga; Kitipumi Chutasmit; Chalermpon Saelow; Ratchadaporn Runcharern; Weerayuth Kaewmok; Nhu Thi Hoa; Ngo Viet Thanh; Borimas Hanboonkunupakarn; James J Callery; Akshaya Kumar Mohanty; James Heaton; Myo Thant; Kripasindhu Gantait; Tarapada Ghosh; Roberto Amato; Richard D Pearson; Christopher G Jacob; Sónia Gonçalves; Mavuto Mukaka; Naomi Waithira; Charles J Woodrow; Martin P Grobusch; Michele van Vugt; Rick M Fairhurst; Phaik Yeong Cheah; Thomas J Peto; Lorenz von Seidlein; Mehul Dhorda; Richard J Maude; Markus Winterberg; Nguyen Thanh Thuy-Nhien; Dominic P Kwiatkowski; Mallika Imwong; Podjanee Jittamala; Khin Lin; Tin Maung Hlaing; Kesinee Chotivanich; Rekol Huy; Caterina Fanello; Elizabeth Ashley; Mayfong Mayxay; Paul N Newton; Tran Tinh Hien; Neena Valecha; Frank Smithuis; Sasithon Pukrittayakamee; Abul Faiz; Olivo Miotto; Joel Tarning; Nicholas P J Day; Nicholas J White; Arjen M Dondorp Journal: Lancet Date: 2020-03-11 Impact factor: 202.731
Authors: Liusheng Huang; Vincent Carey; Jane C Lindsey; Florence Marzan; David Gingrich; Bobbie Graham; Linda Barlow-Mosha; Phionah K Ssemambo; Portia Kamthunzi; Sharon Nachman; Sunil Parikh; Francesca T Aweeka Journal: PLoS One Date: 2017-10-24 Impact factor: 3.240