| Literature DB >> 21082400 |
Abstract
Adipose-derived mesenchymal stem or stromal cells (ASCs) are poised for clinical use in an allogeneic setting. Although ASCs have been shown to be nonimmunogenic by several laboratories, it is advisable for the investigator to confirm this for ASCs used in their studies due to variations in ASC production and the animal models in which they are used. We describe here the use of the mixed lymphocyte reaction (MLR) assay to determine immunogenicity and suppression by ASCs in vitro as well as assessing T cell responses to allogeneic ASC transplantation in vivo. A flow cytometry assay to determine serum antibody titer to transplanted ASCs is also described.Entities:
Mesh：
Year: 2011 PMID： 21082400 DOI： 10.1007/978-1-61737-960-4_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745