| Literature DB >> 21082392 |
Gang Yu1, Xiying Wu, Gail Kilroy, Yuan-Di C Halvorsen, Jeffrey M Gimble, Z Elizabeth Floyd.
Abstract
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Primary adipocytes grown in culture and derived from murine adipose tissue are essential for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stem cells along with a protocol for inducing adipogenesis in this cell population.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21082392 DOI： 10.1007/978-1-61737-960-4_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745