Literature DB >> 21082391

Isolation of human adipose-derived stem cells from lipoaspirates.

Gang Yu1, Z Elizabeth Floyd, Xiying Wu, Yuan-Di C Halvorsen, Jeffrey M Gimble.   

Abstract

Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large-scale procedure suitable for processing >100-ml volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.

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Mesh：

Year:  2011        PMID： 21082391     DOI： 10.1007/978-1-61737-960-4_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  29 in total

1.  A rapid and efficient method for primary culture of human adipose-derived stem cells.

Authors:  Guofang Zeng; Kuan Lai; Jin Li; Yaqin Zou; Haili Huang; Jie Liang; Xudong Tang; Jing Wei; Peihua Zhang
Journal:  Organogenesis       Date:  2013-11-22       Impact factor: 2.500

2.  Enhanced viability and neural differential potential in poor post-thaw hADSCs by agarose multi-well dishes and spheroid culture.

Authors:  Xiaoling Guo; Shanyi Li; Qingshan Ji; Ruiling Lian; Jiansu Chen
Journal:  Hum Cell       Date:  2015-06-09       Impact factor: 4.174

Review 3.  Concise review: adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical implications for tissue engineering therapies in regenerative surgery.

Authors:  Pietro Gentile; Augusto Orlandi; Maria Giovanna Scioli; Camilla Di Pasquali; Ilaria Bocchini; Valerio Cervelli
Journal:  Stem Cells Transl Med       Date:  2012-02-22       Impact factor: 6.940

4.  Interleukin 6 mediates the therapeutic effects of adipose-derived stromal/stem cells in lipopolysaccharide-induced acute lung injury.

Authors:  Shijia Zhang; Svitlana D Danchuk; Ryan W Bonvillain; Beibei Xu; Brittni A Scruggs; Amy L Strong; Julie A Semon; Jeffrey M Gimble; Aline M Betancourt; Deborah E Sullivan; Bruce A Bunnell
Journal:  Stem Cells       Date:  2014-06       Impact factor: 6.277

5.  Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway.

Authors:  Peihua Zhang; Jin Li; Yawei Qi; Yaqing Zou; Li Liu; Xudong Tang; Jianfeng Duan; Hongwei Liu; Guofang Zeng
Journal:  Organogenesis       Date:  2016-05-26       Impact factor: 2.500

6.  Application of adipose-derived stem cells on scleral contact lens carrier in an animal model of severe acute alkaline burn.

Authors:  Ladan Espandar; Delmar Caldwell; Richard Watson; Tomas Blanco-Mezquita; Shijia Zhang; Bruce Bunnell
Journal:  Eye Contact Lens       Date:  2014-07       Impact factor: 2.018

7.  Obesity-associated dysregulation of calpastatin and MMP-15 in adipose-derived stromal cells results in their enhanced invasion.

Authors:  Amy L Strong; Julie A Semon; Thomas A Strong; Tatyana T Santoke; Shijia Zhang; Harris E McFerrin; Jeffrey M Gimble; Bruce A Bunnell
Journal:  Stem Cells       Date:  2012-12       Impact factor: 6.277

8.  Differentiation of Human Adipose-derived Stem Cells along the Keratocyte Lineage In vitro.

Authors:  Shijia Zhang; Ladan Espandar; Kathleen M P Imhof; Bruce A Bunnell
Journal:  J Clin Exp Ophthalmol       Date:  2013-02-27

9.  Safety and Efficacy of Human Adipose-Derived Stromal/Stem Cell Therapy in an Immunocompetent Murine Pressure Ulcer Model.

Authors:  Joanna Bukowska; Andrea Alarcon Uquillas; Xiying Wu; Trivia Frazier; Katarzyna Walendzik; Mikaela Vanek; Dina Gaupp; Bruce A Bunnell; Paul Kosnik; Babak Mehrara; Adam J Katz; Barbara Gawronska-Kozak; Jeffrey M Gimble
Journal:  Stem Cells Dev       Date:  2020-02-12       Impact factor: 3.272

10.  Biodegradable silk catheters for the delivery of therapeutics across anatomical repair sites.

Authors:  Joseph E Brown; Lorenzo Tozzi; Benjamin Schilling; Arta Kelmendi-Doko; April B Truong; Maria J Rodriguez; Eun Seok Gil; Robert Sucsy; Jolene E Valentin; Brian J Philips; Kacey G Marra; J Peter Rubin; David L Kaplan
Journal:  J Biomed Mater Res B Appl Biomater       Date:  2018-04-26       Impact factor: 3.368
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