Adenosquamous versus adenocarcinoma of the pancreas: a population-based outcomes analysis.

BACKGROUND: Pancreatic adenosquamous carcinoma has historically been characterized as having a more aggressive clinical course than ductal adenocarcinoma. The natural history of this disease, however, is essentially unknown.
METHODS: We evaluated the clinical characteristics of all patients with pancreatic adenosquamous carcinoma recorded in the California Cancer Registry 2000-2007 and compared them to those of patients with ductal adenocarcinoma.
RESULTS: Ninety-five patients with pancreatic adenosquamous carcinoma and 14,746 patients with ductal adenocarcinoma were identified. Demographics were similar between subtypes (p > 0.05). Disease stage at presentation was also similar; over 50% of each diagnostic group presented with metastatic disease (p = 0.62). Surgical resection was more common among patients with locoregional adenosquamous carcinoma than adenocarcinoma (p = 0.0004), but rates of adjuvant therapy administration were similar (p > 0.05). The cohorts' median overall survival durations were similar in a Cox proportional hazards model (p = 0.45); overall survival was also similar when only patients with resected disease were considered (p = 0.65). Early stage, resection and receipt of radiation or chemotherapy were favorable independent prognostic factors among patients with adenosquamous carcinoma. The median overall survival duration of patients with resected adenosquamous carcinoma was 12 months (95% CI, 8-52).
CONCLUSIONS: Adenosquamous carcinoma has a natural history similar to that of ductal adenocarcinoma when treated with prevalent clinical patterns of care.

Introduction

Adenosquamous carcinoma (ASC) is a rare pancreatic cancer that is has been suggested to be distinct from pancreatic ductal adenocarcinoma (AC) both histopathologically and clinically.1, 2 Histologically, ASC is distinguished from AC by the presence of both adenocarcinomatous and squamous components.3 Clinically, the disease has been characterized by an extremely poor prognosis, even relative to that of AC—which itself is associated with median overall survival durations as low as 3–6 months among patients with metastatic disease and as high as 24 months among patients with resectable cancers.4, 5 Indeed, the median overall survival duration of patients with localized ASC has been reported to be as low as 6 months following radical tumor resection, with 2-year survival an infrequent event. Patients with advanced ASC treated with palliative intent have fared even worse.5–7

The histopathologic phenotype of ASC is well defined and thus ASC remains a unique diagnostic entity. The clinical significance of this diagnosis is unclear, however, because its natural history is poorly understood. Indeed, the demographics, treatment patterns, and oncologic outcomes of patients with ASC are essentially unknown because all clinical knowledge of the disease has been accumulated from case studies 8–26 and small, single-institution anecdotes—reporting patients compiled over a period of decades—the overwhelming majority of whom had localized disease and were treated with surgery alone.2, 5, 7, 27–31 Given the time, stage, and treatment biases inherent in these previous reports, we hypothesized that the natural history of ASC has been mischaracterized and its clinical significance overstated. We sought to more completely establish the clinical profile of ASC relative to AC and to elucidate any unique characteristics that might influence the design of rational treatment strategies. To these ends, we examined a consecutive series of patients with ASC recorded in a large state cancer registry over a recent 8-year time period. We evaluated demographic and clinical features of ASC, including survival estimates after treatment with prevalent patterns of care, and compared these clinical parameters to those of patients with AC treated in the same recent time period.

Patients and Methods

Cancer Registry

We performed a historical analysis of cases in the California Cancer Registry database (CCR). The CCR is the largest contiguous area, population-based cancer registry in the world, collecting more than 130,000 new cases yearly. Standardized data collection and quality control procedures have been in place since 1988.32, 33 The CCR is part of the National Cancer Institute’s Surveillance, Epidemiology and End Results (SEER) Program. Case reporting is estimated at 99% for the state, and follow-up completion rates exceed 95%.34, 35 The CCR has received the highest level of certification from the North American Association of Central Cancer Registries.36 Data were abstracted from medical records by trained registrars according to standardized protocols.32, 33 Tumor site and histology were coded according to standardized criteria.37

Study Population

Histopathologic diagnoses recorded in the CCR were ascertained by examination of fine needle aspiration or surgical specimens by local pathologists. Pancreatic tumors were identified using the SEER primary site recode 21100. Pancreatic ductal adenocarcinomas were identified by ICDO (third edition) histology codes 8140, 8141, 8142, 8144, 8490, 8500, 8501, 8503, 8504, 8507.37 Adenosquamous carcinomas were identified by histology code 8560. Other non-ductal cancers were expressly excluded. All incident cases recorded between January 2000 and November 2007 for whom complete follow-up data were available through November 2007 were included for analysis.

Recorded data included demographic information, histology, burden of disease at presentation, first treatment history, socioeconomic status, and vital status. Socioeconomic status is denoted as a single index variable using statewide measures of education, income, and occupation from census data, as described previously.38, 39 Quintiles for the socioeconomic status score were used for analysis, with socioeconomic status 1 and 5 denoting the lowest and highest quintiles, respectively.

The criteria used for American Joint Commission on Cancer (AJCC) staging of pancreatic cancer underwent a dramatic revision between the fifth and sixth editions.40 In the CCR, AJCC staging per seventh edition guidelines is available only for cases diagnosed in or after the year 2004. We therefore allocated cases by the SEER summary stage into cohorts with “localized” (no tumor extension or malignant regional lymphadenopathy regardless of tumor size), “regional” (based on the presence of either tumor extension to adjacent viscera or lymph nodes), or “metastatic” disease. Patients with localized or regional disease in whom pancreaticoduodenectomy, distal pancreatectomy, or total pancreatectomy were performed were considered to have undergone an oncologic resection; patients who underwent an oncologic resection who received either chemotherapy or radiation therapy in the first course of treatment were considered to have undergone adjuvant therapy. Hospital registrars contacted cases annually, and CCR staff annually reviewed state death certificates to identify deceased registry cases.

Statistical Analysis

Clinical characteristics were analyzed with Pearson’s chi-square test or Fisher’s exact test for categorical and dichotomous variables and the Student’s t test for comparison of continuous variables. The overall survival duration (in months) was calculated using dates of diagnosis and either death from any cause or last contact. The Kaplan–Meier method was used to generate survival curves. The log-rank test was used to assess differences between survival curves. Multivariate survival analyses were performed using Cox proportional hazards ratios. Fifty-five patients (all of whom had AC) in whom a diagnosis of cancer was made by review of an autopsy report or death certificate were excluded from all survival analyses. All analyses were conducted using SAS 9.2 (SAS Institute, Inc., Cary, NC). Statistical significance was assumed for a two-tailed p value < 0.05.

Results

Demographics of Patients with ASC and AC

Between 2000 and 2007, 24,604 incident cases of pancreatic neoplasm were recorded in the CCR. Of these, 14,746 (59.9%) patients with AC and 95 (0.38%) patients with ASC were included in this analysis. Demographic data for these patients are reported in Table 1. The median age at diagnosis, sex, race, socioeconomic status, and clinical stage of patients with ASC and AC were similar (p > 0.05). The majority of patients with each diagnosis were Caucasian; sex and socioeconomic status were evenly distributed. Over 50% of both groups were found to have metastatic disease upon presentation. In contrast, localized disease was identified in less than 10% of incident cases of each histopathologic subtype.

Table 1

Demographics and treatment of patients with pancreatic adenosquamous carcinoma and ductal adenocarcinoma reported in California, 2000–2007

	Adenosquamous	Adenocarcinoma	p
N, %	95 (0.39)	14,746 (59.9)
Demographic variables
Age, mean (SD)	68.5 (11.8)	68.6 (11.8)	0.9188
Sex, n (%)			0.1565
Male	55 (57.9)	7,462 (50.6)
Female	40 (42.1)	7,284 (49.4)
Ethnicity, n (%)			0.3740
White	72 (75.8)	9,760 (66.2)
Black	5 (5.3)	1,108 (7.5)
Hispanic	11 (11.6)	2,425 (16.5)
Asian	7 (7.4)	1,365 (9.3)
Other	0 (0)	88 (0.6)
SES quintile, n (%)			0.3013
Lowest	10 (10.5)	2,083 (14.1)
Second lowest	22 (23.2)	2,622 (17.8)
Middle	14 (14.7)	3,153 (21.4)
High	23 (24.2)	3,322 (22.5)
Highest	26 (27.4)	3,566 (24.2)
Clinical stage, n (%)a			0.6242
Localized	8 (8.9)	976 (7.0)
Regional	34 (37.8)	4,864 (35.1)
Metastatic	48 (53.3)	8,029 (57.9)
Missing data, n (%)b	5 (5.3)	877 (5.9)
Treatment variables
Any surgery, n (%)a			<0.0001 a
Yes	31 (32.6)	2,428 (16.5)
No	64 (67.4)	12,300 (83.5)
Missing data, n (%)b	0 (0)	18 (0.1)
Any radiation, n (%)a			0.1515a
Yes	20 (21.1)	2,310 (15.7)
No	75	12,422 (84.3)
Missing data, n (%)b	0 (0)	14 (0.1)
Any chemotherapy, n (%)a			0.6786a
Yes	42 (46.2)	6,296 (44.0)
No	49 (53.8)	8,016 (56.0)
Missing data, n (%)b	4 (4.2)	434 (2.9)
Locoregional patients, n evaluated	42	5,838
Onc. resection, n (%)			0.0004
Yes	26 (61.9)	2,071 (35.6)
No	16 (38.1)c	3,750 (64.4)
Type of resection, n (%)			0.1084
PD	18 (69.2)	1,690 (81.6)
Distal panc.	5 (19.2)	181 (8.7)
Total panc.	3 (11.5)	200 (9.7)
Tumor diam. (mm); mean (SD)a	46.3 (19.0)	33.5 (15.1)	0.0001
Missing data, n (%)b	0 (0)	117 (5.7)
Lymph nodes positive, n (%)a			0.8562
Yes	15 (57.7)	1,236 (60.2)
No	11 (42.3)	816 (39.8)
Missing data, n (%)b	0 (0)	19 (0.9)
Adj. chemotherapy, n (%)a			0.9902
Yes	13 (52.0)	1,037 (51.9)
No	12 (48)	962 (48.1)
Missing data, n (%)b	1 (3.8)	72 (3.5)
Adj. radiation, n (%)			0.2876
Yes	12 (46.2)	747 (36.1)
No	14 (53.8)	1,324 (63.9)
Metastatic patients, n evaluated	46	7,832
Pall. chemotherapy, n (%)			0.2397
Yes	24 (52.2)	3,411 (43.6)
No	22 (47.8)	4,421 (56.4)

The numbers in bold are those which are statistically significant, i.e. p < 0.05

For each variable, data was complete unless otherwise specified

SES socioeconomic status, Onc. oncologic, panc. pancreatectomy, Adj. adjuvant, Pall. palliative, diam. diameter, PD pancreaticoduodenectomy

aPercentage and p values refer to patients with complete data

bPercentage of total patients

cAmong patients with locoregional ASC in whom the reason an oncologic resection was not performed was recorded, surgery was not recommended in 12 (two with localized cancers and ten regional), and one patient refused an operation

Treatment Patterns and Pathologic Variables of ASC and AC

Surgery was utilized more frequently for patients with ASC than those with AC, both overall (32.6% vs 16.5%, p < 0.0001) and among patients with locoregional cancers (61.9% vs 35.6%, p = 0.0004) (Table 1). Oncologic procedures performed for patients with ASC included pancreaticoduodenectomy (n = 18), distal (n = 5), and total pancreatectomy (n = 3); the distribution of these operations was similar to that performed for AC (p = 0.11). The mean tumor diameter in resected ASC specimens was larger than that in AC specimens (46.3 vs 33.5 mm, p = 0.0001), but the frequency of positive lymph nodes was similar (57.7 vs 60.2%, p = 0.86).

Overall, radiation (p = 0.15) and chemotherapy (p = 0.68) were administered to similar proportions of patients with ASC and AC. Twelve (46.2%) patients with ASC who underwent an oncologic resection were treated with adjuvant radiation and 13 (52.0%) received chemotherapy. Rates of administration of adjuvant radiation (p = 0.29) and adjuvant chemotherapy (p = 0.99) following resection for locoregional disease did not differ between groups. Likewise, among patients with metastatic disease, the rate of administration of palliative chemotherapy did not differ between patients with ASC and AC (p = 0.24).

Overall Survival of ASC and AC

As a group, the median overall survival duration of all patients with ASC was 4 months (95% CI, 3–6) and was similar to that of all patients with AC (p = 0.41, Fig. 1a). The median overall survival duration of patients with ASC was also similar to that of patients with AC in subpopulations of patients stratified by age, sex, ethnicity, socioeconomic status, clinical stage, and the use of oncologic resection, radiation therapy, and chemotherapy on univariate analysis (p > 0.05, data not shown). Furthermore, the median overall survival duration of all patients with ASC was similar to that of all patients with AC in a Cox proportional hazards model after adjustment for age, gender, ethnicity, socioeconomic status, stage of disease, and first treatment strategy [hazard ratio (HR), 1.091; 95% CI, 0.870–1.367; p = 0.45] (Table 2). Finally, when only patients with locoregional cancers who underwent resection were considered, the median overall survival duration of patients with each histopathologic diagnosis were similar after adjustment for age, gender, ethnicity, socioeconomic status, clinical stage, tumor size, lymphatic involvement, and the receipt of adjuvant therapy (HR, 0.886; 95% CI, 0.530–1.482; p = 0.65) (Table 3).

Fig. 1

a Overall survival of all patients with pancreatic adenosquamous carcinoma and ductal adenocarcinoma reported in California, 2000–2007. Dashed line, adenosquamous carcinoma (ASC); solid line, pancreatic ductal adenocarcinoma (AC). b Overall survival of patients with localized or regional adenosquamous carcinoma stratified by resection status. Dashed line, resected (R); solid line, not resected (NR)

Table 2

Cox proportional hazards model for overall survival of all patients with pancreatic adenosquamous carcinoma and ductal adenocarcinoma reported in California, 2000–2007

	HR	95% CI	p
Histologic subtype
AC	1.000 (referent)
ASC	1.091	0.870–1.367	0.4509
Age	1.010	1.009–1.012	<0.0001
Gender
Male	1.000 (referent)
Female	0.951	0.918–0.986	0.006
Ethnicity
Caucasian	1.000 (referent)
Black	1.035	0.966–1.108	0.3290
Hispanic	0.955	0.906–1.006	0.0802
Asian	0.922	0.866–0.981	0.0108
Socioeconomic status	0.966	0.953–0.980	<0.0001
Clinical stage
Localized	1.000 (referent)
Regional	1.275	1.177–1.382	<0.0001
Metastatic	2.293	2.117–2.484	<0.0001
Oncologic resection
No	1.000 (referent)
Yes	0.444	0.419–0.472	<0.0001
Any radiation
No	1.000 (referent)
Yes	0.887	0.840–0.936	<0.0001
Any chemotherapy
No	1.000 (referent)
Yes	0.508	0.488–0.528	<0.0001

The numbers in bold are those which are statistically significant, i.e. p < 0.05

HR hazard ratio for death, 95% CI 95% confidence interval

Table 3

Cox proportional hazards model for overall survival of patients with resected locoregional pancreatic adenosquamous carcinoma and ductal adenocarcinoma reported in California, 2000–2007

	HR	95% CI	p
Histologic subtype
AC	1.000 (referent)
ASC	0.886	0.530–1.482	0.6454
Age	1.008	1.003–1.014	0.0043
Gender
Male	1.000 (referent)
Female	0.998	0.893–1.116	0.9740
Ethnicity
Caucasian	1.000 (referent)
Black	1.095	0.865–1.387	0.4514
Hispanic	1.043	0.884–1.231	0.6150
Asian	1.007	0.818–1.241	0.9445
Socioeconomic status	0.945	0.905–0.987	0.0112
Clinical stage
Localized	1.000 (referent)
Regional	1.300	1.067–1.583	0.0091
Tumor diameter	1.009	1.005–1.012	<0.0001
Lymphatic involvement
No	1.000 (referent)
Yes	1.386	1.212–1.585	<0.0001
Adjuvant radiation
No	1.000 (referent)
Yes	0.791	0.679–0.922	0.0027
Adjuvant chemotherapy
No	1.000 (referent)
Yes	0.651	0.561–0.756	<0.0001

The numbers in bold are those which are statistically significant, i.e. p < 0.05

HR hazard ratio for death, 95% CI 95% confidence interval

Favorable Prognostic Factors among Patients with ASC

Among all patients with ASC, favorable prognostic factors on univariate analysis included early clinical stage (p < 0.0001), oncologic resection (p < 0.0001), receipt of radiation (p < 0.0001), and receipt of chemotherapy (p < 0.0233). In a Cox proportional hazards model, each of these factors remained independently significant (Table 4).

Table 4

Cox proportional hazards model for overall survival of all patients with pancreatic adenosquamous carcinoma reported in California, 2000–2007

	HR	95% CI	p
Age	1.012	0.986–1.038	0.3730
Gender
Male	1.000 (referent)
Female	0.905	0.536–1.528	0.7088
Ethnicity
Caucasian	1.000 (referent)
Black	0.703	0.244–2.023	0.5135
Hispanic	0.890	0.390–2.032	0.7815
Asian	0.655	0.244–1.755	0.3998
Socioeconomic status	0.936	0.765–1.145	0.5915
Clinical stage
Localized	1.000 (referent)
Regional	2.717	0.781–9.451	0.1161
Metastatic	4.690	1.445–15.216	0.0101
Oncologic resection
No	1.000 (referent)
Yes	0.369	0.183–0.747	0.0056
Any radiation
No	1.000 (referent)
Yes	0.474	0.242–0.927	0.0292
Any chemotherapy
No	1.000 (referent)
Yes	0.530	0.300–0.935	0.0285

The numbers in bold are those which are statistically significant, i.e. p < 0.05

HR hazard ratio for death, 95% CI 95% confidence interval

Separate multivariate models were not constructed for patients with locoregional or metastatic ASC due to relatively small numbers in each of these subgroups. Among patients with locoregional ASC, however, those who underwent an oncologic resection had a median survival duration of 12 months (95% CI, 8–52) compared with 5 months (95% CI, 1–12) for those who did not, and the survival curves were significantly different (p = 0.018) (Fig. 1b). A significant difference in survival could not be demonstrated between patients with resected locoregional ASC who did and did not receive adjuvant therapy (p = 0.09 overall). Eight patients with locoregional ASC survived longer than 2 years, four of whom survived over 5 years. Each of these 5-year survivors underwent surgery and received adjuvant therapy.

Among patients with metastatic ASC, patients who received chemotherapy had a more favorable median survival duration (4.5 months; 95% CI, 3–6 months) than patients who did not (2 months; 95% CI, 1–3 months; p = 0.04).

Discussion

ASC and AC share a similar histologic 3 and molecular 41 profile. ASC, however, has long been characterized as having a natural history distinctly more aggressive than that of AC. This has led some to question the role of aggressive treatment strategies for patients with this disease.2, 5, 7, 27 The clinical significance of this rare diagnosis relative to AC is unclear, however, because the oncologic behavior of ASC has been described only by case studies and small, retrospective surgical series reporting patients with early stage cancers (Table 5). Moreover, no prior case–control studies or population-based analyses have been performed to definitively establish clinical differences between ASC and AC. In this, the largest study of ASC reported to date, we used a large cancer registry to evaluate the clinical features and oncologic outcomes of patients with this diagnosis. Using a relatively unbiased dataset, we characterize the natural history of ASC and show that ASC is no more inherently aggressive than AC. Indeed, we demonstrate that patients with these two diagnoses have a similar natural history when treated using prevalent patterns of modern clinical practice.

Table 5

Published case reports and clinical series of patients with pancreatic adenosquamous carcinoma, 1990–2010

Author (ref.)	Number	Resected, n (%)	Median age (years)	Adjuvant treatment, n	Median OS resected, months	Median OS unresected, months
Skafida8	1	1 (100)	70	1 CTX	6	NA
Lampropoulos9	1	1 (100)	72	1 CXRT	24	NA
Voong27	38	38 (100)	68	19 CTX 19 CXRT	10.9	NA
Kobayashi10	1	0 (0)	72	NA	NA	3
Smoot7	23	12 (52)	67a	5 CXRT	13.1	4.8
Hsu5	12	7 (58)	71	5 CTX	6.51	NR
Jamali11	1	1 (100)	75	1 CTX	6	NA
Alwaheeb12	1	1 (100)	45	NR	NR	NA
Inoue13	1	0 (0)	61	NA	NA	0.83
Murakami14	2	2 (100)	54	1 CTX 1 CXRT	4.5	NA
Rahemtullah28	14	2 (14)	70a	NR	13	4
Kardon29	25	13 (52)	65a	5 CTX	11.3	3.0
Yamaue15	1	1 (100)	63	1 CXRT	40	NA
Yavuz16	2	2 (100)	50	NR	36, NR	NA
Komatsuda17	1	1 (100)	67	0	6	NA
Aranha18	2	2 (100)	57	2 CXRT	13.5	NA
Madura2	6	6 (100)	64a	3 CXRT	5	NA
Nabae19	2	2 (100)	67	1 RT 1 NR	6.5	NA
Lozano20	3	2 (67)	59a	3 CXRTb	NR	NR
Myung21	1	1 (100)	64	0	4	NA
Kuji22	1	1 (100)	73	0	2	NA
Campman23	1	1 (100)	65	NR	NR	NA
Onoda24	1	1 (100)	64	1 CTX	3	NA
Makiyama25	1	1 (100)	58	0	18	NA
Tanaka26	1	1 (100)	48	1 CTX	7	NA
Motojima30	6	3 (50)	67c	NR	7	NR
Yamaguchi31	8	8 (100)	56a	0	5.5	NA

NR not recorded, NA not applicable, CTX chemotherapy, CXRT chemoradiation, RT radiation, OS overall survival

aMean

bNeoadjuvant chemoradiation

cResected only

ASC has been reported to represent up to 4% of pancreatic neoplasms, but in the largest series of specimens analyzed at autopsy, ASC was identified in only 0.9%.42, 43 In this analysis of a large tumor registry, we found a diagnosis of ASC in approximately 0.4% of 24,604 patients with newly documented pancreatic malignancies recorded between 2000 and 2007. This is remarkably similar to the rate of 0.5% identified in a recent 16-year survey of the State of Michigan Tumor Registry.44

Like patients with AC, most of the patients with ASC presented late in their natural history. Indeed, over 50% of patients analyzed in this study initially presented with synchronous distant metastases. Among patients treated surgically, those with ASC had larger tumors than those with AC; however, a larger proportion of patients with locoregional ASC underwent resection than that with AC, and resected ASC specimens were associated with a similar high rate of regional lymphatic involvement—approximately 60%—as AC tumors. Together, these findings reveal that—although considerably rarer—ASC presents at a similar (albeit advanced) stage as AC and suggest that the two diagnoses share a common biologic behavior prior to diagnosis and treatment.

Stage-specific treatment algorithms for patients with AC are reasonably well-established.45 In contrast, the absolute infrequency of ASC has prohibited the development of standardized treatment protocols for this disease. Indeed, even the treatment of patients with early stage ASC remains controversial, due to reportedly dismal survival rates seemingly regardless of intervention.5, 7 In a recent systematic review of prior reports, 39 patients with ASC who underwent surgery for non-metastatic disease had a median survival duration of 6.8 months (range, 4.6–9) and a 1-year survival rate of 25.5%.6 In two recent single-institution series, overall survival of resected patients was somewhat more favorable. Among 38 resected patients from Johns Hopkins, the median overall survival duration was 10.9 months from diagnosis.27 In another series from the Mayo Clinic, patients who underwent R0 or R1 resection had a median survival duration of 14.4 months and 8 months, respectively, compared to 4.8 months among patients treated without an operation.7 The patients in each group were not described, however, suggesting that patients who did not undergo resection had advanced disease, prior comorbidities, a depressed performance status, or a combination of these factors.

The efficacy of non-operative therapies among patients with ASC has not been rigorously evaluated. Only one prior study has examined the utility of adjuvant chemoradiation for patients with this disease. In that small, retrospective series, 19 (50%) patients who underwent postoperative chemoradiation had a more favorable median overall survival than 19 (50%) patients who did not (13.6 months v. 8.6 months, p = 0.005).27 Although adjuvant chemoradiation was found to be the only significant prognostic factor with respect to overall survival on univariate analysis, the analysis suffered from clear selection bias. No studies have specifically studied the effects of systemic chemotherapy when administered in the adjuvant setting, nor its role as palliative therapy for patients with metastatic disease.

In this study, treatment of patients with ASC by surgical resection was associated with a more favorable overall survival relative to no resection, after adjustment for multiple clinical factors including disease stage. Moreover, the overall survival duration of patients with locoregional ASC who underwent surgery was similar to that of patients with locoregional AC who underwent resection in the same time period. Together with the recent single-institution data from high-volume pancreatic treatment centers,7, 27 these data suggest that resection is a reasonable therapeutic approach for patients with ASC in whom a margin-negative resection can be performed safely.

The role of non-operative therapies for patients with ASC is less clear. Although we could demonstrate no association between the administration of adjuvant radiation or chemotherapy on the survival of patients with locoregional ASC following resection, it is interesting that of the only six 5-year survivors with ASC reported to date (four in this series and two in the Johns Hopkins series 27), all received surgery and adjuvant therapy. Among patients with metastatic ASC, patients who received chemotherapy had a longer overall survival duration (4.5 vs 2 months) than patients who did not. The significance of this finding is uncertain, however, because individual performance status—the most influential factor with regard to the administration of anticancer therapy among patients with advanced pancreatic malignancy—was not recorded in the CCR.46 The absence of recorded performance status represents a fundamental limitation of this and other analyses of pancreatic malignancies using large, population-based datasets.

Two other limitations of this study are particularly noteworthy. Although attempts have been made to identify characteristic molecular fingerprints that may effectively distinguish between ASC and AC, the molecular profile of these two tumors are similar.41 Therefore, ASC must be distinguished from AC histopathologically. A strict diagnosis of ASC requires that a malignant squamous component represent at least 30% of a routinely sectioned adenocarcinoma.3, 29 This arbitrary cutoff has introduced ambiguity to the diagnosis of ASC that reflects both the absence of standardization in histopathologic methods used to process surgical specimens and the subjectivity with which they are evaluated. Indeed, when 38 surgical specimens initially diagnosed as ASC at Johns Hopkins were re-evaluated by a single pathologist, 12 (32%) failed to meet strict criteria for the disease.27 Significantly, although the presence of any squamous component was associated with poor prognosis in the Johns Hopkins study relative to a historic control group of patients with AC, the proportion of the squamous component was not associated with overall survival. The rationale for the strict 30% cutoff is therefore unclear, and several investigators have proposed eliminating this criterion altogether.29

It is also possible that some diagnoses were coded incorrectly in the CCR; however, all diagnoses recorded therein were validated by histopathologic or cytopathologic analysis. Moreover, accuracy of the histopathologic diagnoses recorded in large databases has been evaluated and compared with independent histologic review, with favorable results.47, 48 Nonetheless, the accuracy associated with the diagnosis of ASC may not be as favorable due to the stringent diagnostic requirements for this disease. A further potential for misclassification may exist among patients with advanced cancer treated non-operatively, for whom a large surgical specimen for histopathologic evaluation is absent. The extent to which our conclusions are influenced by this issue is unknown.

In summary, we conclude that ASC is an extremely rare subtype of pancreatic cancer that shares many clinical characteristics—including biologic behavior and overall prognosis—with AC. In this population, the overall survival duration of all patients with ASC and AC were similar after adjustment for multiple clinical factors, including stage at presentation and first treatment strategy. These data therefore refute prior suggestions that ASC is inherently more aggressive than AC and imply that a nihilistic view toward patients with ASC must be avoided. Absent the ability to perform prospective studies to determine the response of ASC to individual therapies, and given the molecular, histopathologic and clinical similarity of these diseases, we recommend the use of aggressive, stage-specific, multidisciplinary treatment protocols developed for AC.