Chizuka Sakai1, Yumi Hoshino, Yusuke Sato, Eimei Sato. 1. Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan. chizuka@bios.tohoku.ac.jp
Abstract
PURPOSE: To ascertain whether metaphase II (MII) spindle shape influences oocyte competence, we examined the meiotic spindle organization in in vivo ovulated (IVO) oocytes and in spontaneously matured or follicle stimulating hormone (FSH)-induced oocytes. METHODS: FSH-induced oocytes matured in Waymouth's MB752/1 or human tubal fluid (HTF) media and oocytes matured spontaneously in the basal medium were obtained, and spindles were detected by immunofluorescence. To evaluate the fertilization-associated differences in spindle morphology, we performed in vitro fertilization and analysed integrin mRNA expression. RESULTS: The distance between the pericentriolar materials (PCMs) in oocytes matured under all conditions was initially more, but it reduced gradually and increased again thereafter. Therefore, oocytes exhibiting a reduction in the distance between PCMs had the highest development rate to blastocyst in each condition. CONCLUSION: These results indicate that the 'maturation competence' of MII oocytes can be evaluated on the basis of the distance between PCMs.
PURPOSE: To ascertain whether metaphase II (MII) spindle shape influences oocyte competence, we examined the meiotic spindle organization in in vivo ovulated (IVO) oocytes and in spontaneously matured or follicle stimulating hormone (FSH)-induced oocytes. METHODS: FSH-induced oocytes matured in Waymouth's MB752/1 or human tubal fluid (HTF) media and oocytes matured spontaneously in the basal medium were obtained, and spindles were detected by immunofluorescence. To evaluate the fertilization-associated differences in spindle morphology, we performed in vitro fertilization and analysed integrin mRNA expression. RESULTS: The distance between the pericentriolar materials (PCMs) in oocytes matured under all conditions was initially more, but it reduced gradually and increased again thereafter. Therefore, oocytes exhibiting a reduction in the distance between PCMs had the highest development rate to blastocyst in each condition. CONCLUSION: These results indicate that the 'maturation competence' of MII oocytes can be evaluated on the basis of the distance between PCMs.
Authors: E A Almeida; A P Huovila; A E Sutherland; L E Stephens; P G Calarco; L M Shaw; A M Mercurio; A Sonnenberg; P Primakoff; D G Myles; J M White Journal: Cell Date: 1995-06-30 Impact factor: 41.582