| Literature DB >> 21081272 |
Hua Kuang1, Shuge Zhao, Wei Chen, Wei Ma, Qianqian Yong, Liguang Xu, Libing Wang, Chuanlai Xu.
Abstract
A novel, rapid DNA detection method based on fluorescence quenching of quantum dots (QDs) by gold nanoparticles (GNPs) through polymerase chain reaction (PCR) was developed. In proof-of-concept experiments, the length of the amplicon DNA ranging from 152 to 1003 base pairs (bp) could be determined based on quenched fluorescence intensity with 136 bp as the lower limit of effective range. And the real sample detections were also achieved successfully by this developed method. Therefore, this DNA detection method has the potential to be the powerful gene diagnostic tool. Copyright ÂMesh：
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Year: 2010 PMID： 21081272 DOI： 10.1016/j.bios.2010.10.043
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618