PURPOSE: This study aims to develop and characterize the trafficking of a dual-modal agent that identifies primary draining or sentinel lymph node (LN). PROCEDURE: Herein, a dual-reporting silica-coated iron oxide nanoparticle (SCION) is developed. Nude mice were imaged by magnetic resonance (MR) and optical imaging and axillary LNs were harvested for histological analysis. Trafficking through lymphatics was observed with intravital and ex vivo confocal microscopy of popliteal LNs in B6-albino, CD11c-EYFP, and lys-EGFP transgenic mice. RESULTS: In vivo, SCION allows visualization of LNs. The particle's size and surface functionality play a role in its passive migration from the intradermal injection site and its minimal uptake by CD11c+ dendritic cells and CD169+ and lys+ macrophages. CONCLUSIONS: After injection, SCION passively migrates to LNs without macrophage uptake and then can be used to image LN(s) by MRI and fluorescence. Thus, SCION can potentially be developed for use in sentinel node resections or for intralymphatic drug delivery.
PURPOSE: This study aims to develop and characterize the trafficking of a dual-modal agent that identifies primary draining or sentinel lymph node (LN). PROCEDURE: Herein, a dual-reporting silica-coated iron oxide nanoparticle (SCION) is developed. Nude mice were imaged by magnetic resonance (MR) and optical imaging and axillary LNs were harvested for histological analysis. Trafficking through lymphatics was observed with intravital and ex vivo confocal microscopy of popliteal LNs in B6-albino, CD11c-EYFP, and lys-EGFP transgenic mice. RESULTS: In vivo, SCION allows visualization of LNs. The particle's size and surface functionality play a role in its passive migration from the intradermal injection site and its minimal uptake by CD11c+ dendritic cells and CD169+ and lys+ macrophages. CONCLUSIONS: After injection, SCION passively migrates to LNs without macrophage uptake and then can be used to image LN(s) by MRI and fluorescence. Thus, SCION can potentially be developed for use in sentinel node resections or for intralymphatic drug delivery.
Authors: Sungjee Kim; Yong Taik Lim; Edward G Soltesz; Alec M De Grand; Jaihyoung Lee; Akira Nakayama; J Anthony Parker; Tomislav Mihaljevic; Rita G Laurence; Delphine M Dor; Lawrence H Cohn; Moungi G Bawendi; John V Frangioni Journal: Nat Biotechnol Date: 2003-12-07 Impact factor: 54.908
Authors: Tobias Junt; E Ashley Moseman; Matteo Iannacone; Steffen Massberg; Philipp A Lang; Marianne Boes; Katja Fink; Sarah E Henrickson; Dmitry M Shayakhmetov; Nelson C Di Paolo; Nico van Rooijen; Thorsten R Mempel; Sean P Whelan; Ulrich H von Andrian Journal: Nature Date: 2007-10-14 Impact factor: 49.962
Authors: A Bumb; M W Brechbiel; P L Choyke; L Fugger; A Eggeman; D Prabhakaran; J Hutchinson; P J Dobson Journal: Nanotechnology Date: 2008-08-20 Impact factor: 3.874
Authors: Susanta K Sarkar; Ambika Bumb; Xufeng Wu; Kem A Sochacki; Peter Kellman; Martin W Brechbiel; Keir C Neuman Journal: Biomed Opt Express Date: 2014-03-14 Impact factor: 3.732
Authors: Anja Ostrowski; Daniel Nordmeyer; Alexander Boreham; Cornelia Holzhausen; Lars Mundhenk; Christina Graf; Martina C Meinke; Annika Vogt; Sabrina Hadam; Jürgen Lademann; Eckart Rühl; Ulrike Alexiev; Achim D Gruber Journal: Beilstein J Nanotechnol Date: 2015-01-23 Impact factor: 3.649
Authors: Xianjin Cui; Domokos Mathe; Noémi Kovács; Ildikó Horváth; Maite Jauregui-Osoro; Rafael Torres Martin de Rosales; Gregory E D Mullen; Wilson Wong; Yong Yan; Dirk Krüger; Andrei N Khlobystov; Maria Gimenez-Lopez; Mariann Semjeni; Krisztián Szigeti; Dániel S Veres; Haizhou Lu; Ignacio Hernández; William P Gillin; Andrea Protti; Katalin Kis Petik; Mark A Green; Philip J Blower Journal: Bioconjug Chem Date: 2015-08-14 Impact factor: 4.774
Authors: Jyothi U Menon; Praveen K Gulaka; Madalyn A McKay; Sairam Geethanath; Li Liu; Vikram D Kodibagkar Journal: Theranostics Date: 2012-12-31 Impact factor: 11.556
Authors: Mijung Jang; Young Il Yoon; Yong Soo Kwon; Tae-Jong Yoon; Hak Jong Lee; Sung Il Hwang; Bo La Yun; Sun Mi Kim Journal: Korean J Radiol Date: 2014-07-09 Impact factor: 3.500