Literature DB >> 21079837

Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes.

Evelyne Weber1, Alexander Seifert, Mihaela Antonovici, Christopher Geinitz, Jürgen Pleiss, Vlada B Urlacher.   

Abstract

A minimal enriched P450 BM3 library was screened for the ability to oxidize inert cyclic and acyclic alkanes. The F87A/A328V mutant was found to effectively hydroxylate cyclooctane, cyclodecane and cyclododecane. F87V/A328F with high activity towards cyclooctane hydroxylated acyclic n-octane to 2-(R)-octanol (46% ee) with high regioselectivity (92%).

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Year:  2010        PMID： 21079837     DOI： 10.1039/c0cc02924f

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  13 in total

1.  Discovery of potent parthenolide-based antileukemic agents enabled by late-stage P450-mediated C-H functionalization.

Authors:  Joshua N Kolev; Kristen M O'Dwyer; Craig T Jordan; Rudi Fasan
Journal:  ACS Chem Biol       Date:  2013-11-08       Impact factor: 5.100

2.  Stabilization of the Reductase Domain in the Catalytically Self-Sufficient Cytochrome P450BM3 by Consensus-Guided Mutagenesis.

Authors:  Gloria Saab-Rincón; Hanan Alwaseem; Valeria Guzmán-Luna; Leticia Olvera; Rudi Fasan
Journal:  Chembiochem       Date:  2018-02-12       Impact factor: 3.164

Review 3.  Cytochrome P450: taming a wild type enzyme.

Authors:  Sang Taek Jung; Ryan Lauchli; Frances H Arnold
Journal:  Curr Opin Biotechnol       Date:  2011-03-14       Impact factor: 9.740

4.  A single active-site mutation of P450BM-3 dramatically enhances substrate binding and rate of product formation.

Authors:  Donovan C Haines; Amita Hegde; Baozhi Chen; Weiqiang Zhao; Muralidhar Bondlela; John M Humphreys; David A Mullin; Diana R Tomchick; Mischa Machius; Julian A Peterson
Journal:  Biochemistry       Date:  2011-09-06       Impact factor: 3.162

5.  Aldehyde and Ketone Synthesis by P450-Catalyzed Oxidative Deamination of Alkyl Azides.

Authors:  Simone Giovani; Hanan Alwaseem; Rudi Fasan
Journal:  ChemCatChem       Date:  2016-07-26       Impact factor: 5.686

6.  Structure-guided directed evolution of highly selective p450-based magnetic resonance imaging sensors for dopamine and serotonin.

Authors:  Eric M Brustad; Victor S Lelyveld; Christopher D Snow; Nathan Crook; Sang Taek Jung; Francisco M Martinez; Timothy J Scholl; Alan Jasanoff; Frances H Arnold
Journal:  J Mol Biol       Date:  2012-05-30       Impact factor: 5.469

7.  Enhancing the efficiency and regioselectivity of P450 oxidation catalysts by unnatural amino acid mutagenesis.

Authors:  Joshua N Kolev; Jacqueline M Zaengle; Rajesh Ravikumar; Rudi Fasan
Journal:  Chembiochem       Date:  2014-04-01       Impact factor: 3.164

8.  P450-catalyzed intramolecular sp3 C-H amination with arylsulfonyl azide substrates.

Authors:  Ritesh Singh; Melanie Bordeaux; Rudi Fasan
Journal:  ACS Catal       Date:  2014-01-06       Impact factor: 13.084

9.  Controlled oxidation of remote sp3 C-H bonds in artemisinin via P450 catalysts with fine-tuned regio- and stereoselectivity.

Authors:  Kaidong Zhang; Brian M Shafer; Matthew D Demars; Harry A Stern; Rudi Fasan
Journal:  J Am Chem Soc       Date:  2012-11-02       Impact factor: 15.419

10.  Selective allylic hydroxylation of acyclic terpenoids by CYP154E1 from Thermobifida fusca YX.

Authors:  Anna M Bogazkaya; Clemens J von Bühler; Sebastian Kriening; Alexandrine Busch; Alexander Seifert; Jürgen Pleiss; Sabine Laschat; Vlada B Urlacher
Journal:  Beilstein J Org Chem       Date:  2014-06-13       Impact factor: 2.883
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