Literature DB >> 21079352

Reducing acyl migration during purification of 2-arachidonoylglycerol from biological samples before gas chromatography mass spectrometry analysis.

Sei Higuchi1, Keiichi Irie, Takafumi Nakano, Yuya Sakamoto, Yoshiharu Akitake, Maiko Araki, Makiko Ohji, Riyo Furuta, Mai Katsuki, Ryuji Yamaguchi, Kiyoshi Matsuyama, Kenji Mishima, Kenichi Mishima, Katsunori Iwasaki, Michihiro Fujiwara.   

Abstract

Endocannabinoid 2-arachidonoylglycerol (2-AG) regulates several important physiological processes in the brain. 2-AG is commonly quantified by gas chromatography mass spectrometry after an initial purification step. The most precise and rapid purification utilizes C(18) solid-phase extraction, but quantification problems can arise with acyl migration from 2-AG to 1-arachidonoylglycerol. We found that extraction with methanol promoted this migration, but acetone and diethyl ether (Et(2)O) did not. Acetone and Et(2)O were used to develop a purification method for the direct determination of 2-AG.

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Year:  2010        PMID： 21079352     DOI： 10.2116/analsci.26.1199

Source DB:  PubMed          Journal:  Anal Sci        ISSN： 0910-6340            Impact factor:   2.081


  6 in total

1.  Estimation of reference intervals of five endocannabinoids and endocannabinoid related compounds in human plasma by two dimensional-LC/MS/MS.

Authors:  Flaminia Fanelli; Valentina D Di Lallo; Ilaria Belluomo; Rosaria De Iasio; Margherita Baccini; Elena Casadio; Daniela Ibarra Gasparini; Michelangelo Colavita; Alessandra Gambineri; Gabriele Grossi; Valentina Vicennati; Renato Pasquali; Uberto Pagotto
Journal:  J Lipid Res       Date:  2011-12-14       Impact factor: 5.922

2.  Endocannabinoids as potential biomarkers: It's all about pre-analytics.

Authors:  Daniel Kratz; Dominique Thomas; Robert Gurke
Journal:  J Mass Spectrom Adv Clin Lab       Date:  2021-11-10

3.  Determination of the Endocannabinoids Anandamide and 2-Arachidonoyl Glycerol with Gas Chromatography-Mass Spectrometry: Analytical and Preanalytical Challenges and Pitfalls.

Authors:  Christian Lanz; Johan Mattsson; Felix Stickel; Jean-Francois Dufour; Rudolf Brenneisen
Journal:  Med Cannabis Cannabinoids       Date:  2018-06-12

4.  Hypothalamic 2-arachidonoylglycerol regulates multistage process of high-fat diet preferences.

Authors:  Sei Higuchi; Keiichi Irie; Ryuji Yamaguchi; Mai Katsuki; Maiko Araki; Makiko Ohji; Kazuhide Hayakawa; Shohei Mishima; Yoshiharu Akitake; Kiyoshi Matsuyama; Kenji Mishima; Kenichi Mishima; Katsunori Iwasaki; Michihiro Fujiwara
Journal:  PLoS One       Date:  2012-06-21       Impact factor: 3.240

5.  Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia.

Authors:  Mette M B Nielsen; Kate L Lambertsen; Bettina H Clausen; Morten Meyer; Dhaka R Bhandari; Søren T Larsen; Steen S Poulsen; Bernhard Spengler; Christian Janfelt; Harald S Hansen
Journal:  Sci Rep       Date:  2016-12-22       Impact factor: 4.379

6.  The Ratio of 2-AG to Its Isomer 1-AG as an Intrinsic Fine Tuning Mechanism of CB1 Receptor Activation.

Authors:  Klaudia Dócs; Zoltán Mészár; Sándor Gonda; Attila Kiss-Szikszai; Krisztina Holló; Miklós Antal; Zoltán Hegyi
Journal:  Front Cell Neurosci       Date:  2017-02-20       Impact factor: 5.505
  6 in total

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