Literature DB >> 21078937

An A643V amino acid substitution in Upc2p contributes to azole resistance in well-characterized clinical isolates of Candida albicans.

Samantha J Hoot1, Adam R Smith, Ryan P Brown, Theodore C White.   

Abstract

The Candida albicans Upc2p transcription factor regulates ERG11, encoding the target of azole drugs. Gain-of-function mutations that contribute to resistance were recently identified in a series of sequential clinical isolates (N. Dunkel, T. T. Liu, K. S. Barker, R. Homayouni, J. Morschhauser, and P. D. Rogers, Eukaryot. Cell 7:1180-1190, 2008). In the present study, UPC2 was sequenced from a matched set of 17 isolates. An A643V substitution was present in all of the isolates in the series that overexpressed ERG11. Azole susceptibility, ergosterol levels, and expression of ERG genes were elevated in the A643V clinical isolates and in reconstructed strains.

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Year:  2010        PMID： 21078937      PMCID： PMC3028767          DOI： 10.1128/AAC.00995-10

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  23 in total

1.  Regulatory mechanisms controlling expression of the DAN/TIR mannoprotein genes during anaerobic remodeling of the cell wall in Saccharomyces cerevisiae.

Authors:  N E Abramova; B D Cohen; O Sertil; R Kapoor; K J Davies; C V Lowry
Journal:  Genetics       Date:  2001-03       Impact factor: 4.562

2.  Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus.

Authors:  T C White
Journal:  Antimicrob Agents Chemother       Date:  1997-07       Impact factor: 5.191

3.  Resistance mechanisms in clinical isolates of Candida albicans.

Authors:  Theodore C White; Scott Holleman; Francis Dy; Laurence F Mirels; David A Stevens
Journal:  Antimicrob Agents Chemother       Date:  2002-06       Impact factor: 5.191

4.  Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.

Authors:  R B Wilson; D Davis; A P Mitchell
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

5.  An A643T mutation in the transcription factor Upc2p causes constitutive ERG11 upregulation and increased fluconazole resistance in Candida albicans.

Authors:  Clemens J Heilmann; Sabrina Schneider; Katherine S Barker; P David Rogers; Joachim Morschhäuser
Journal:  Antimicrob Agents Chemother       Date:  2009-11-02       Impact factor: 5.191

6.  Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae.

Authors:  Cintia Hongay; Nan Jia; Martin Bard; Fred Winston
Journal:  EMBO J       Date:  2002-08-01       Impact factor: 11.598

7.  Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast.

Authors:  Lisa J Wilcox; Dina A Balderes; Brook Wharton; Arthur H Tinkelenberg; Govinda Rao; Stephen L Sturley
Journal:  J Biol Chem       Date:  2002-06-20       Impact factor: 5.157

8.  A mutation in a purported regulatory gene affects control of sterol uptake in Saccharomyces cerevisiae.

Authors:  J H Crowley; F W Leak; K V Shianna; S Tove; L W Parks
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490

9.  Genome-wide expression profile analysis reveals coordinately regulated genes associated with stepwise acquisition of azole resistance in Candida albicans clinical isolates.

Authors:  P David Rogers; Katherine S Barker
Journal:  Antimicrob Agents Chemother       Date:  2003-04       Impact factor: 5.191

10.  Isolation and characterization of the Saccharomyces cerevisiae SUT1 gene involved in sterol uptake.

Authors:  S Bourot; F Karst
Journal:  Gene       Date:  1995-11-07       Impact factor: 3.688
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  43 in total

Review 1.  Polymerase chain reaction-based assays for the diagnosis of invasive fungal infections.

Authors:  Themistoklis K Kourkoumpetis; Beth Burgwyn Fuchs; Jeffrey J Coleman; Athanasios Desalermos; Eleftherios Mylonakis
Journal:  Clin Infect Dis       Date:  2012-02-23       Impact factor: 9.079

2.  SREBP-dependent triazole susceptibility in Aspergillus fumigatus is mediated through direct transcriptional regulation of erg11A (cyp51A).

Authors:  Sara J Blosser; Robert A Cramer
Journal:  Antimicrob Agents Chemother       Date:  2011-10-17       Impact factor: 5.191

3.  UPC2 is universally essential for azole antifungal resistance in Candida albicans.

Authors:  Erin M Vasicek; Elizabeth L Berkow; Stephanie A Flowers; Katherine S Barker; P David Rogers
Journal:  Eukaryot Cell       Date:  2014-03-21

4.  Induction of Candida albicans drug resistance genes by hybrid zinc cluster transcription factors.

Authors:  Sabrina Schneider; Joachim Morschhäuser
Journal:  Antimicrob Agents Chemother       Date:  2014-11-10       Impact factor: 5.191

Review 5.  The development of fluconazole resistance in Candida albicans - an example of microevolution of a fungal pathogen.

Authors:  Joachim Morschhäuser
Journal:  J Microbiol       Date:  2016-02-27       Impact factor: 3.422

6.  UPC2A is required for high-level azole antifungal resistance in Candida glabrata.

Authors:  Sarah G Whaley; Kelly E Caudle; John-Paul Vermitsky; Sean G Chadwick; Geoffrey Toner; Katherine S Barker; Scott E Gygax; P David Rogers
Journal:  Antimicrob Agents Chemother       Date:  2014-05-27       Impact factor: 5.191

7.  Crystallization and preliminary X-ray crystallographic analysis of sterol transcription factor Upc2 from Saccharomyces cerevisiae.

Authors:  Subin Ha; Junsen Tong; Huiseon Yang; Hyung-Seop Youn; Soo Hyun Eom; Young Jun Im
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2013-01-31

8.  Comparison of sterol import under aerobic and anaerobic conditions in three fungal species, Candida albicans, Candida glabrata, and Saccharomyces cerevisiae.

Authors:  Martin Zavrel; Sam J Hoot; Theodore C White
Journal:  Eukaryot Cell       Date:  2013-03-08

9.  Milbemycins: more than efflux inhibitors for fungal pathogens.

Authors:  Luis Vale Silva; Maurizio Sanguinetti; Patrick Vandeputte; Riccardo Torelli; Bertrand Rochat; Dominique Sanglard
Journal:  Antimicrob Agents Chemother       Date:  2012-12-03       Impact factor: 5.191

10.  Ascorbic acid inhibition of Candida albicans Hsp90-mediated morphogenesis occurs via the transcriptional regulator Upc2.

Authors:  Frédérique Van Hauwenhuyse; Alessandro Fiori; Patrick Van Dijck
Journal:  Eukaryot Cell       Date:  2014-08-01
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