SUMMARY: BACKGROUND: Umbilical cord blood (CB) is widely used for hematopoietic stem cell transplantation and holds promise for the development of innovative medicinal products. In order to find out whether the conditions for collection and storage before processing might have an impact on the quality of CB preparations, viability and the clonogenic potential were assessed. METHODS: CB was collected under field conditions. Flow cytometry was used to determine leukocytes, CD34/CD45+ cells, viability, and nucleated red blood cells (NRBC). Clonogenic activity was determined using isolated mononuclear cells (MNC). RESULTS: Neither plasma citrate concentrations nor storage temperature (within 24 h) affected cell viability or colony formation. After storage for 49-80 h, leukocyte viability declined by about 16% compared to CB stored up to 24 h. In contrast, the clonogenic activity and CD34/CD45+ cell content were not affected. A higher gestational age was associated with a lower yield of clonogenic activity compared to midterm deliveries. NRBC varied widely (median 7.3%; range 0.63-17.3%) without relation to gestational age or colony formation. There was a close correlation between the percentage of viable CD34/CD45+ cells and colony formation (r = 0.77 for CFU-GM; r = 0.75 for CFU-C). CONCLUSIONS: The content of viable CD34/CD45+ cells represents the clonogenic activity of CB preparations. Therefore, determination of viable CD34/CD45+ cells should be generally performed as a routine quality control assay.
SUMMARY: BACKGROUND: Umbilical cord blood (CB) is widely used for hematopoietic stem cell transplantation and holds promise for the development of innovative medicinal products. In order to find out whether the conditions for collection and storage before processing might have an impact on the quality of CB preparations, viability and the clonogenic potential were assessed. METHODS: CB was collected under field conditions. Flow cytometry was used to determine leukocytes, CD34/CD45+ cells, viability, and nucleated red blood cells (NRBC). Clonogenic activity was determined using isolated mononuclear cells (MNC). RESULTS: Neither plasma citrate concentrations nor storage temperature (within 24 h) affected cell viability or colony formation. After storage for 49-80 h, leukocyte viability declined by about 16% compared to CB stored up to 24 h. In contrast, the clonogenic activity and CD34/CD45+ cell content were not affected. A higher gestational age was associated with a lower yield of clonogenic activity compared to midterm deliveries. NRBC varied widely (median 7.3%; range 0.63-17.3%) without relation to gestational age or colony formation. There was a close correlation between the percentage of viable CD34/CD45+ cells and colony formation (r = 0.77 for CFU-GM; r = 0.75 for CFU-C). CONCLUSIONS: The content of viable CD34/CD45+ cells represents the clonogenic activity of CB preparations. Therefore, determination of viable CD34/CD45+ cells should be generally performed as a routine quality control assay.
Authors: Mitchell S Cairo; Elizabeth L Wagner; John Fraser; Geoff Cohen; Carmella van de Ven; Shelly L Carter; Nancy A Kernan; Joanne Kurtzberg Journal: Transfusion Date: 2005-06 Impact factor: 3.157
Authors: Gerard Michel; Vanderson Rocha; Sylvie Chevret; William Arcese; Kah-Wah Chan; Alexandra Filipovich; Tsuneo A Takahashi; Marcus Vowels; Juan Ortega; Pierre Bordigoni; Peter J Shaw; Isaac Yaniv; Alexandra Machado; Pedro Pimentel; Franca Fagioli; Amparo Verdeguer; Jean Pierre Jouet; Blanca Diez; Euripedes Ferreira; Ricardo Pasquini; Joseph Rosenthal; Eric Sievers; Chiara Messina; Anna Paola Iori; Federico Garnier; Irina Ionescu; Franco Locatelli; Eliane Gluckman Journal: Blood Date: 2003-08-14 Impact factor: 22.113
Authors: John Patterson; Cally H Moore; Emily Palser; Jason C Hearn; Daniela Dumitru; Holli A Harper; Ivan N Rich Journal: J Transl Med Date: 2015-03-18 Impact factor: 5.531
Authors: Juan Pellico; Ana V Lechuga-Vieco; Elena Almarza; Andrés Hidalgo; Cristina Mesa-Nuñez; Irene Fernández-Barahona; Juan A Quintana; Juan Bueren; Jose A Enríquez; Jesús Ruiz-Cabello; Fernando Herranz Journal: Sci Rep Date: 2017-10-16 Impact factor: 4.379